Identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets by polyclonal antibody-based enzyme-linked immunosorbent assay

被引：22

作者：

Asensio, L ^{[1
]}

González, I ^{[1
]}

Rodríguez, MA ^{[1
]}

Mayoral, B ^{[1
]}

López-Calleja, I ^{[1
]}

Hernández, PE ^{[1
]}

García, T ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr & Bromatol Higene & Tecnol Alimentos 3, E-28040 Madrid, Spain

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2003年 / 51卷 / 05期

关键词：

fish species identification; ELISA; Epinephelus guaza; Polyprion americanus; Lates niloticus;

D O I：

10.1021/jf026020x

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

An indirect enzyme-linked immunosorbent assay (ELISA) has been developed for the species identification of grouper (Epinephelus guaza), wreck fish (Polyprion americanus), and Nile perch (Lates niloticus) fillets. The assay was performed in two different formats, microtiter plates and immunostick tubes, and uses polyclonal antibodies raised in rabbits against muscle-soluble proteins of grouper (anti-GSP), wreck fish (anti-WSP), and Nile perch (anti-PSP). The antibodies were made species-specific by blocking them with the heterologous soluble muscle proteins. Immunorecognition of polyclonal antibodies adsorbed to their specific fish samples was made with swine antirabbit immunoglobulins conjugated to the enzyme horseradish peroxidase. Subsequent enzymatic conversion of the substrate allowed unequivocal identification of the species studied.

引用

页码：1169 / 1172

页数：4

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