Immunostick ELISA for detection of cow's milk in ewe's milk and cheese using a monoclonal antibody against bovine beta-casein

被引：27

作者：

Anguita, G ^{[1
]}

Martin, R ^{[1
]}

Garcia, T ^{[1
]}

Morales, P ^{[1
]}

Haza, AI ^{[1
]}

Gonzalez, I ^{[1
]}

Sanz, B ^{[1
]}

Hernandez, PE ^{[1
]}

机构：

[1] UNIV COMPLUTENSE MADRID,FAC VET,DEPT NUTR & BROMATOL 3,E-28040 MADRID,SPAIN

来源：

JOURNAL OF FOOD PROTECTION | 1996年 / 59卷 / 04期

关键词：

monoclonal antibodies; cow's milk detection; ELISA;

D O I：

10.4315/0362-028X-59.4.436

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An immunostick enzyme-linked immunosorbent assay (ELISA) has been developed for the rapid detection of cow's milk in ewe's milk or cheese, The assay uses a monoclonal antibody (AH4) produced against bovine beta-casein for the detection of cow's milk or cheese bound to the paddles of immunostick tubes. This immunostick ELISA allows the visual identification of ewe's milk containing more than 1% of cow's milk or cheese samples containing more than 0.5% of cow's cheese.

引用

页码：436 / 437

页数：2

共 9 条

[1] [Anonymous], 1988, Antibodies: A Laboratory Manual
[2] QUANTITATIVE FRACTIONATION OF CASEIN MIXTURES BY FAST PROTEIN LIQUID-CHROMATOGRAPHY
DAVIES, DT
LAW, AJR
[J]. JOURNAL OF DAIRY RESEARCH, 1987, 54 (03) : 369 - 376
[3] DETECTION OF BOVINE-MILK IN OVINE MILK BY AN INDIRECT ENZYME-LINKED-IMMUNOSORBENT-ASSAY
GARCIA, T
MARTIN, R
RODRIGUEZ, E
MORALES, P
HERNANDEZ, PE
SANZ, B
[J]. JOURNAL OF DAIRY SCIENCE, 1990, 73 (06) : 1489 - 1493
[4] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY
KOHLER, G
MILSTEIN, C
[J]. NATURE, 1975, 256 (5517) : 495 - 497
[5] RAPID, SENSITIVE 2-SITE ELISA FOR DETECTION OF COWS MILK IN GOATS OR EWES MILK USING MONOCLONAL-ANTIBODIES
LEVIEUX, D
VENIEN, A
[J]. JOURNAL OF DAIRY RESEARCH, 1994, 61 (01) : 91 - 99
[6] DETECTION OF COWS MILK IN EWES MILK AND CHEESE BY A SANDWICH ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)
RODRIGUEZ, E
MARTIN, R
GARCIA, T
GONZALEZ, I
MORALES, P
SANZ, B
HERNANDEZ, PE
[J]. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1993, 61 (02) : 175 - 180
[7] POLYCLONAL ANTIBODIES WITH PREDETERMINED SPECIFICITY AGAINST BOVINE ALPHA-S1-CASEIN - APPLICATION TO THE DETECTION OF BOVINE-MILK IN OVINE MILK AND CHEESE
ROLLAND, MP
BITRI, L
BESANCON, P
[J]. JOURNAL OF DAIRY RESEARCH, 1993, 60 (03) : 413 - 420
[8] SELF-MANAGEMENT OF DIETARY COMPLIANCE IN CELIAC-DISEASE BY MEANS OF ELISA HOME TEST TO DETECT GLUTEN
SKERRITT, JH
HILL, AS
[J]. LANCET, 1991, 337 (8738) : 379 - 382
[9] VONSAUER S, 1991, ARCH LEBENSMITTELHYG, V42, P133

← 1 →