Alpha-1 Antitrypsin-Deficient Macrophages Have Increased Matriptase-Mediated Proteolytic Activity

被引:17
作者
Krotova, Karina [1 ]
Marek, George W. [1 ]
Wang, Rejean L. [1 ]
Aslanidi, George [2 ]
Hoffman, Brad E. [2 ]
Khodayari, Nazli [1 ]
Rouhani, Farshid N. [1 ]
Brantly, Mark L. [1 ]
机构
[1] Univ Florida, Dept Med, Div Pulm Crit Care & Sleep Med, Gainesville, FL 32610 USA
[2] Univ Florida, Dept Pediat, Gainesville, FL 32610 USA
关键词
macrophages; alpha-1 antitrypsin deficiency; extracellular matrix degradation; matriptase; matrix metalloproteinase-14; CONNECTIVE-TISSUE MATRICES; UNFOLDED PROTEIN RESPONSE; ALPHA-1-ANTITRYPSIN DEFICIENCY; ALPHA(1)-ANTITRYPSIN DEFICIENCY; PLASMINOGEN-ACTIVATOR; ALVEOLAR MACROPHAGES; PULMONARY-EMPHYSEMA; TUMOR-GROWTH; IN-VITRO; DEGRADATION;
D O I
10.1165/rcmb.2016-0366OC
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Alpha-1 antitrypsin (AAT) deficiency-associated emphysema is largely attributed to insufficient inhibition of neutrophil elastase released from neutrophils. Correcting AAT levels using augmentation therapy only slows disease progression, and that suggests a more complex process of lung destruction. Because alveolar macrophages (M phi) express AAT, we propose that the expression and intracellular accumulation of mutated Z-AAT (the most common mutation) compromisesM. function and contributes to emphysema development. Extracellular matrix (ECM) degradation is a hallmark of emphysema pathology. In this study, M phi from individuals with Z-AAT (Z-M phi) have greater proteolytic activity onECMthan do normalM phi. This abnormal Z-M phi activity is not abrogated by supplementation with exogenous AAT and is likely the result of cellular dysfunction induced by intracellular accumulation of Z-AAT. Using pharmacologic inhibitors, we show that several classes of proteases are involved in matrix degradation by Z-M phi. Importantly, compared with normal M phi, the membranebound serine protease, matriptase, is present in Z-M phi at higher levels and contributes to their proteolytic activity on ECM. In addition, we identified matrix metalloproteinase (MMP)-14, a membraneanchored metalloproteinase, as a novel substrate for matriptase, and showed that matriptase regulates the levels of MMP-14 on the cell surface. Thus, high levels of matriptase may contribute to increased ECM degradation by Z-M phi, both directly and through MMP-14 activation. In summary, the expression of Z-AAT in M phi confers increased proteolytic activity on ECM. This proteolytic activity is not rescued by exogenous AAT supplementation and could thus contribute to augmentation resistance in AAT deficiency-associated emphysema.
引用
收藏
页码:238 / 247
页数:10
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