Detection of Newcastle disease virus with quantum dots-resonance light scattering system

被引：23

作者：

Ma, Qiang ^{[1
]}

Yu, Wei ^{[1
]}

Su, Xingguang ^{[1
]}

机构：

[1] Jilin Univ, Dept Analyt Chem, Coll Chem, Changchun 130012, Jilin, Peoples R China

来源：

TALANTA | 2010年 / 82卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Resonance light scattering; CdTe QDs; Newcastle disease virus; Immunocomplex; PROBES; SERUM; CDTE; DNA; HYBRIDIZATION; NANOCRYSTALS; PROTEINS; ASSAY; CDSE;

D O I：

10.1016/j.talanta.2010.03.056

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive QDs-based RLS assay method for the detection of Newcastle disease virus (NDV) antibody has been developed. CdTe quantum dots (QDs) were conjugated with Newcastle disease virus and used as RLS-based probes to detect NDV antibody. The electrostatic interaction between CdTe QDs and NDV resulted in enhanced resonance light scattering (RLS) signal characterized at 555 nm. Upon the addition of NDV antibody, QDs-NDV formed dispersive immunocomplex that can decrease the RLS signal. The decreased RLS intensity at 555 nm (Delta I-RLS) was linearly proportional to the concentration of NDV antibody (Canti-NDV) in the range of 0.5-50 ng/mL, with correlation coefficient of 0.974 and detection limit of 0.1 ng/mL under the optimization conditions. The proposed method was applied to the determination of NDV antibody in spiked samples with satisfactory results. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：51 / 55

页数：5

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