PACAP-27 radioimmunoassay:: Description and application of a novel method

被引：11

作者：

Nemeth, J.

Jakab, B.

Jozsa, R.

Hollosy, T.

Tamas, A.

Lubics, A.

Lengvari, I.

Kiss, P.

Oberritter, Zs.

Horvath, B.

Szilvassy, Z.

Reglodi, D.

机构：

[1] Univ Pecs, Hungarian Acad Sci, Dept Pharmacol & Pharmacotherapy, Neuropharmacol Res Grp, H-7624 Pecs, Hungary

[2] Univ Pecs, Hungarian Acad Sci, Dept Anat, Neurohumoral Regulat Res Grp, H-7624 Pecs, Hungary

[3] Univ Pecs, Dept Pediat, H-7624 Pecs, Hungary

[4] Univ Debrecen, Dept Pharmacol & Pharmacotherapy, H-4019 Debrecen, Hungary

来源：

JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY | 2007年 / 273卷 / 02期

关键词：

D O I：

10.1007/s10967-007-6862-3

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Pituitary adenylate cyclase activating polypeptide (PACAP) is a widely distributed neuropeptide that has two molecular forms with 38 and 27 amino acid residues. The aim of the present study was to develop a new, highly specific PACAP-27 assay to investigate the quantitative distribution of PACAP-27 in the central nervous system of various vertebrate species applying the same technical and experimental conditions. Our results show that the antiserum used turned to be PACAP-27 specific. The average ID50 value was 51.5 +/- 3.6 fmol/ml and the detection limit was 2 fmol/ml. PACAP-27 immunoreactivity was present in the examined brain areas, with highest concentration in the rat diencephalon and telencephalon. Swine and pigeon brain also contained significant amount of PACAP-27. Our results confirm the previously described data showing that PACAP-38 is the dominant form of PACAP in vertebrates, since PACAP-38 levels exceeded those of PACAP-27 in all examined brain areas. Furthermore, our study describes for the first time, the comparative quantitative distribution of PACAP-27 and-38 in the swine and pigeon brain.

引用

页码：327 / 332

页数：6

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