Production of a non-toxic site-directed mutant of Clostridium perfringens ε-toxin which induces protective immunity in mice

被引：56

作者：

Oyston, PCF ^{[1
]}

Payne, DW ^{[1
]}

Havard, HL ^{[1
]}

Williamson, ED ^{[1
]}

Titball, RW ^{[1
]}

机构：

[1] Def Evaluat & Res Agcy, CBD Porton Down, Salisbury SP4 0JQ, Wilts, England

来源：

MICROBIOLOGY-SGM | 1998年 / 144卷

关键词：

Clostridium perfringens; vaccine; epsilon-toxin; site-directed mutants;

D O I：

10.1099/00221287-144-2-333

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A panel of ten site-directed mutants of Clostridium perfringens epsilon-toxin was generated. All of the mutated proteins expressed in Escherichia coli were recognized in immunoblots by a neutralizing mAb raised against wild-type native epsilon-toxin. The cytotoxicity of the site-directed mutated toxins was assayed in vitro against MDCK cells. One mutation resulting in loss of activity in the assay was identified. This non-toxic protein was derived by substituting a proline for the histidine at residue 106 of the toxin. Immunization of mice with the non-toxic mutated epsilon-toxin resulted in the induction of a specific antibody response and immunized mice were protected against 1000 LD50 doses of wild-type recombinant epsilon-toxin.

引用

页码：333 / 341

页数：9

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