Sol-gel-derived thick-film amperometric immunosensors

被引:207
作者
Wang, J [1 ]
Pamidi, PVA
Rogers, KR
机构
[1] New Mexico State Univ, Dept Chem & Biochem, Las Cruces, NM 88003 USA
[2] US EPA, Natl Exposure Res Lab, Las Vegas, NV 89139 USA
关键词
D O I
10.1021/ac971093e
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Sol-gel processing is used for the first time for the preparation of electrochemical immunosensors. One-step sensor fabrication, based on the coupling of sol-gel and screen-printing technologies, is employed. A low-temperature cured ink is prepared by dispersion of rabbit immunoglobulin G (RIgG), graphite powder, and a binder in the sol-gel solution. The enzyme-labeled antibody can readily diffuse toward the encapsulated antigen, which retains its binding properties, and the association reaction is easily detected at the dispersed graphite surface. Use of anti-RIgG labeled with alkaline phosphatase, naphthyl phosphate as the substrate, and amperometric detection at +400 mV (vs Ag/AgCl) results in a low detection limit of 5 ng/mL (32 pM) for the solution antigen. Tailoring the porosity of the ceramic-carbon matrix can be used for tuning the assay performance. The high sensitivity, low cost, durability, and simplicity of the new single-use immunosensors make them well suited for various onsite applications.
引用
收藏
页码:1171 / 1175
页数:5
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