Bubble dynamics in double-stranded DNA

被引：87

作者：

Altan-Bonnet, G

Libchaber, A

Krichevsky, O

机构：

[1] Rockefeller Univ, Ctr Studies Phys & Biol, New York, NY 10021 USA

[2] Ben Gurion Univ Negev, Dept Phys, IL-84105 Beer Sheva, Israel

来源：

PHYSICAL REVIEW LETTERS | 2003年 / 90卷 / 13期

关键词：

D O I：

10.1103/PhysRevLEtt.90.138101

中图分类号：

O4 [物理学];

学科分类号：

0702 ;

摘要：

We report the first measurement of the dynamics of bubble formation in double-stranded DNA. Fluctuations of fluorescence of a synthetic DNA construct, internally tagged with a fluorophore and a quencher, are monitored by fluorescence correlation spectroscopy. The relaxation dynamics follow a multistate relaxation kinetics, with a characteristic time scale of 50 mus. A simple model of bubble dynamics based on constant zipping-unzipping rates is proposed to account for our experimental data. The role of different secondary structures stabilizing the open bubble is tested.

引用

页数：4

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