Antisense inhibition of the photosystem I antenna protein Lhca4 in Arabidopsis thaliana

被引:51
作者
Zhang, H [1 ]
Goodman, HM
Jansson, S
机构
[1] Texas Tech Univ, Dept Biol Sci, Lubbock, TX 79409 USA
[2] Harvard Univ, Sch Med, Dept Genet, Boston, MA 02114 USA
[3] Massachusetts Gen Hosp, Dept Mol Biol, Boston, MA 02114 USA
[4] Umea Univ, Dept Plant Physiol, S-90187 Umea, Sweden
关键词
D O I
10.1104/pp.115.4.1525
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The function of Lhca4, a gene encoding the photosystem I type IV chlorophyll a/b-binding protein complex in Arabidopsis, was investigated using antisense technology. Lhca4 protein was reduced in a number of mutant lines and abolished in one. The inhibition of protein was not correlated with the inhibition of mRNA. No depletion of Lhca1 was observed, but the low-temperature fluorescence emission spectrum was drastically altered in the mutants. The emission maximum was blue-shifted by 6 nm, showing that chlorophyll molecules bound to Lhca4 are responsible for most of the long-wavelength fluorescence emission. Some mutants also showed an unexplainable delay in flowering time and an increase in seed weight.
引用
收藏
页码:1525 / 1531
页数:7
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