Immune cells in bronchoalveolar lavage in peripheral lung cancer - Analysis of 140 cases

被引:25
作者
Domagala-Kulawik, J
Guzman, J
Costabel, U
机构
[1] Ruhrlandklin, Dept Pneumol & Allergol, D-45239 Essen, Germany
[2] Med Acad, Dept Pneumol & Allergol, Warsaw, Poland
[3] Ruhr Univ Bochum, D-4630 Bochum, Germany
关键词
bronchoalveolar lavage; lung cancer; immunology; lymphocyte subsets; eosinophils;
D O I
10.1159/000068414
中图分类号
R56 [呼吸系及胸部疾病];
学科分类号
摘要
Background: Changes in the bronchoalveolar lavage fluid (BALF) cell profile may reflect immunologic reactions of the lung in pulmonary malignancies. Objective: Our aim was to analyse the BALF cell profile in peripheral lung cancer. Methods: This study was a retrospective analysis of BALF samples containing tumor cells from patients with confirmed peripheral lung cancer (1140 cases). Routine analysis and lymphocyte phenotyping by an immunoperoxidase technique with the monoclonal antibodies CD3, CD20, CD4, CD8, CD57, CD1, CD25, CD71 and HLA-DR were performed. The data were compared with those from healthy persons. Results: We found a significantly lower proportion of macrophages (60%), and significantly elevated proportions of lymphocytes (24%) and neutrophils (13%) in cases with cancer when compared with controls. The proportion of eosinophils was higher in the cancer patients (2.4%), though not significantly. Regarding the lymphocyte phenotypes, we found a significantly elevated proportion of T cells (86%), a relatively low CD4/CD8 ratio (1.7 +/- 1.3), and a relatively higher percentage of HLA-DR+ lymphocytes (6.6%) in patients with cancer. There were no significant differences between smokers and nonsmokers in the cancer patients. In more than half of the cases, a positive staining for epithelial membrane antigen was found. Conclusion: Our observations confirm the possible participation of lymphocytes (activated and suppressor subtypes) and eosinophils in the response against tumor in peripheral airways. Copyright (C) 2003 S. Karger AG, Basel.
引用
收藏
页码:43 / 48
页数:6
相关论文
共 40 条
[31]  
ROUSSEL E, 1995, CANCER IMMUNOL IMMUN, V41, P1
[32]  
Samoszuk M, 1997, HISTOL HISTOPATHOL, V12, P807
[33]  
SEMENZATO G, 1992, RESPIRATION, V59, P44
[34]   Augmentation of impaired tumoricidal function in alveolar macrophages from lung cancer patients by cocultivation with allogeneic, but not autologous lymphocytes [J].
Siziopikou, KP ;
Ahn, MC ;
Casey, L ;
Silver, M ;
Harris, JE ;
Braun, DP .
CANCER IMMUNOLOGY IMMUNOTHERAPY, 1997, 45 (01) :29-36
[35]  
STRIZ I, 1993, EUR RESPIR J, V6, P1287
[36]   PULMONARY MACROPHAGES SUPPRESS THE PROLIFERATION AND CYTOTOXICITY OF TUMOR-INFILTRATING LYMPHOCYTES [J].
SWISHER, SG ;
KIERTSCHER, SM ;
GOLUB, SH ;
HOLMES, EC ;
ROTH, MD .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 1993, 8 (05) :486-492
[37]   LYMPHOCYTIC SUBPOPULATION PROFILES IN BRONCHOALVEOLAR LAVAGE FLUID AND PERIPHERAL-BLOOD FROM TOBACCO AND MARIJUANA SMOKERS [J].
WALLACE, JM ;
OISHI, JS ;
BARBERS, RG ;
SIMMONS, MS ;
TASHKIN, DP .
CHEST, 1994, 105 (03) :847-852
[38]   AN ASSOCIATION BETWEEN SPUTUM EOSINOPHILIA AND CARCINOMA OF THE LUNG - A STUDY OF 549 PATIENTS [J].
WRIGHT, P ;
KELLY, P ;
YAZBECK, J ;
CLANCY, L ;
HEALY, T .
IRISH JOURNAL OF MEDICAL SCIENCE, 1994, 163 (11) :492-493
[39]   PHENOTYPES OF LYMPHOCYTES INFILTRATING NONSMALL CELL LUNG-CANCER TISSUES AND ITS VARIATION WITH HISTOLOGICAL TYPES OF CANCER [J].
YOSHINO, I ;
YANO, T ;
MURATA, M ;
MIYAMOTO, M ;
ISHIDA, T ;
SUGIMACHI, K ;
KIMURA, G ;
NOMOTO, K .
LUNG CANCER, 1993, 10 (1-2) :13-19
[40]   OLIGOCLONAL LYMPHOCYTES-T INFILTRATING HUMAN LUNG-CANCER TISSUES [J].
YOSHINO, I ;
YANO, T ;
YOSHIKAI, Y ;
MURATA, M ;
SUGIMACHI, K ;
KIMURA, G ;
NOMOTO, K .
INTERNATIONAL JOURNAL OF CANCER, 1991, 47 (05) :654-658