Application of enzyme field effect transistors for fast detection of total glycoalkaloids content in potatoes

The possibility to use enzyme field effect transistors for determination of total glycoalkaloids content in real potato samples, without any pre-treatment, has been shown. A measurement protocol for such detection has been developed. The possibility and effectiveness of total glycoalkaloids determination in juice samples from different varieties of potatoes with biosensor based on butyryl cholinesterase was evaluated using two biosensor approaches: the method of standard additions and calibration curve application. A good correlation between results obtained with two biosensor approaches and routinely used method have been demonstrated. The biosensor methods proposed are cheap, easy to operate and requires a short analysis time compared to traditional methods for glycoalkaloids determination. (C) 2004 Elsevier B.V. All rights reserved.