Probing single secretory vesicles with capillary electrophoresis

被引：100

作者：

Chiu, DT

Lillard, SJ

Scheller, RH

Zare, RN ^{[1
]}

Rodriguez-Cruz, SE

Williams, ER

Orwar, O

Sandberg, M

Lundqvist, JA

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[2] Stanford Univ, Howard Hughes Med Inst, Dept Cellular & Mol Physiol, Stanford, CA 94305 USA

[3] Univ Calif Berkeley, Dept Chem, Berkeley, CA 94720 USA

[4] Gothenburg Univ, Dept Chem, SE-41296 Gothenburg, Sweden

[5] Gothenburg Univ, Dept Anat & Cell Biol, SE-41390 Gothenburg, Sweden

来源：

SCIENCE | 1998年 / 279卷 / 5354期

关键词：

D O I：

10.1126/science.279.5354.1190

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Secretory vesicles obtained from the atrial gland of the gastropod mollusk Aplysia californica were chemically analyzed individually with a combination of optical trapping, capillary electrophoresis separation, and a laser-induced fluorescence detection. With the use of optical trapping, a single Vesicle that had attoliters (10(-18) liters) of volume was introduced into the tapered inlet of a separation capillary, Once the vesicle was injected, it was lysed, and its components were fluorescently labeled with naphthalene-2,3-dicarboxaldehyde before separation, The resultant electropherograms indicated distinct variations in the contents of single vesicles.

引用

页码：1190 / 1193

页数：4