Widespread occurrence of Mycobacterium tuberculosis DNA from 18th-19th century Hungarians

被引:69
作者
Fletcher, HA
Donoghue, HD
Holton, J
Pap, I
Spigelman, M
机构
[1] UCL, Dept Med Microbiol, London W1T 4JF, England
[2] Hungarian Natl Hist Museum, Dept Anthropol, H-1083 Budapest, Hungary
关键词
Mycobacterium tuberculosis; ancient DNA; PCR; paleomicrobiology; history of disease;
D O I
10.1002/ajpa.10114
中图分类号
Q98 [人类学];
学科分类号
030303 ;
摘要
A large number (265) of burials from 1731-1838 were discovered in sealed crypts of the Dominican Church, Vac, Hungary in 1994. Many bodies were naturally mummified, so that both soft tissues and bones were available. Contemporary archives enabled the determination of age at death, and the identification of family groups. In some cases, symptoms before death were described and, occasionally, occupation. Initial radiological examination of a small number of individuals had indicated calcified lung lesions and demonstrable acid-fast bacteria suggestive of tuberculosis infection. Tuberculosis was endemic in 18th-19th century Europe, so human remains should contain detectable Mycobacterium tuberculosis complex (MTB) DNA, enabling comparisons with modern isolates. Therefore, a comprehensive examination of 168 individuals for the presence of MTB DNA was undertaken. Specific DNA amplification methods for MTB showed that 55% of individuals were positive and that the incidence varied according to age at death and sampling site in the body. Radiographs were obtained from 27 individuals and revealed an association between gross pathology and the presence of MTB DNA. There was an inverse relationship between PCR positivity and MTB target sequence size. In some cases, the preservation of MTB DNA was excellent, and several target gene sequences could be detected from the same sample. This information, combined with MTB DNA sequencing data and molecular typing techniques, will enable us to study the past epidemiology of TB infection, and extends the timeframe for studying changes in molecular fingerprints. Am J Phys Anthropol 120:144-152, 2003. (C) 2003 Wiley-Liss, Inc.
引用
收藏
页码:144 / 152
页数:9
相关论文
共 47 条
[1]   Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat [J].
Abu Al-Soud, W ;
Rådström, P .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (12) :4463-4470
[2]  
[Anonymous], TUBERCULOSIS
[3]   PRE-COLUMBIAN TUBERCULOSIS IN NORTHERN CHILE - MOLECULAR AND SKELETAL EVIDENCE [J].
ARRIAZA, BT ;
SALO, W ;
AUFDERHEIDE, AC ;
HOLCOMB, TA .
AMERICAN JOURNAL OF PHYSICAL ANTHROPOLOGY, 1995, 98 (01) :37-45
[4]   Mycobacterium tuberculosis complex DNA in ancient human bones [J].
Baron, H ;
Hummel, S ;
Herrmann, B .
JOURNAL OF ARCHAEOLOGICAL SCIENCE, 1996, 23 (05) :667-671
[5]   RAPID AND SIMPLE METHOD FOR PURIFICATION OF NUCLEIC-ACIDS [J].
BOOM, R ;
SOL, CJA ;
SALIMANS, MMM ;
JANSEN, CL ;
WERTHEIMVANDILLEN, PME ;
VANDERNOORDAA, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1990, 28 (03) :495-503
[6]   DNA from Mycobacterium-tuberculosis complex identified in North American, pre-Columbian human skeletal remains (Archaeology and human biology) [J].
Braun, M ;
Cook, DC ;
Pfeiffer, S .
JOURNAL OF ARCHAEOLOGICAL SCIENCE, 1998, 25 (03) :271-277
[7]   THE ENVELOPE OF MYCOBACTERIA [J].
BRENNAN, PJ ;
NIKAIDO, H .
ANNUAL REVIEW OF BIOCHEMISTRY, 1995, 64 :29-63
[8]   Impact of social interactions in the community on the transmission of tuberculosis in a high incidence area [J].
Classen, CN ;
Warren, R ;
Richardson, M ;
Hauman, JH ;
Gie, RP ;
Ellis, JHP ;
van Helden, PD ;
Beyers, N .
THORAX, 1999, 54 (02) :136-140
[9]  
Cooper A, 2000, SCIENCE, V289, P1139
[10]   USE OF POLYMERASE CHAIN-REACTION FOR RAPID DIAGNOSIS OF TUBERCULOSIS [J].
COUSINS, DV ;
WILTON, SD ;
FRANCIS, BR ;
GOW, BL .
JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (01) :255-258