AMPA receptor protein in developing rat brain: Glutamate receptor-1 expression and localization change at regional, cellular, and subcellular levels with maturation

被引:86
作者
Martin, LJ [1 ]
Furuta, A
Blackstone, CD
机构
[1] Johns Hopkins Univ, Sch Med, Dept Pathol, Div Neuropathol, Baltimore, MD 21205 USA
[2] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA
[3] Johns Hopkins Univ, Sch Med, Dept Neurol, Baltimore, MD 21205 USA
关键词
excitotoxicity; glutamate receptor; ion channel; Purkinje cell; receptor clustering; striatum;
D O I
10.1016/S0306-4522(97)00411-9
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We tested the hypothesis that the regional, cellular, and synaptic localizations of the glutamate receptor 1 (GluR1) subunit of the alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor are regulated developmentally in rat brain. By immunoblotting, GluR1 was first detected in whole brain at embryonic day E15.5, and levels increased progressively during late embryonic (E20) and early postnatal (P2-P11) days. Regionally, GluR1 increased in cerebral cortex but decreased in striatum with postnatal maturation. These changes occurred in the presence of increased presynaptic maturation, as determined by synaptophysin detection. By immunocytochemistry, distinct cellular populations showed different temporal profiles of GluR1 expression during postnatal maturation. The neocortex and hippocampus showed a progressive maturation-related enrichment of GluR1, whereas the striatum showed a gradual reduction in GluR1 during maturation. In cerebellum GluR1 protein was expressed transiently at restricted times postnatally by granule cells (P0-P11) and Purkinje cells (P13-P19), but by P21 and thereafter these neurons had sparse GluR1 immunoreactivity. By immunoelectron microscopy, GluR1 was found in neurites, specifically in both dendritic and axon terminal components of developing synapses. GluR1 was clustered at the plasma membrane of apparent growth cone appositions, neuronal cell bodies, and dendrites of developing neurons. The presence of GluR1 at presynaptic sites dissipated with synaptic maturation, as GluR1 became confined to the somatodendritic compartment as maturation progressed. We conclude that the regional expression as well as the cellular and synaptic localizations of the GluR1 are developmentally regulated and are different in immature and mature brain. Differences in glutamate receptor expression and synaptic localization in immature and mature brain may be relevant to the phenomenon that the perinatal and adult brain differ in their regional vulnerability to hypoxia-ischemia and excitotoxicity. (C) 1998 IBRO. Published by Elsevier Science Ltd.
引用
收藏
页码:917 / 928
页数:12
相关论文
共 45 条
[1]  
AOKI C, 1994, J NEUROSCI, V14, P5202
[2]   MICROTUBULE-ASSOCIATED PROTEIN 2(MAP-2) - A SENSITIVE MARKER OF SEIZURE-RELATED BRAIN-DAMAGE [J].
BALLOUGH, GPH ;
MARTIN, LJ ;
CANN, FJ ;
GRAHAM, JS ;
SMITH, CD ;
KLING, CE ;
FORSTER, JS ;
PHANN, S ;
FILBERT, MG .
JOURNAL OF NEUROSCIENCE METHODS, 1995, 61 (1-2) :23-32
[3]   DEVELOPMENT OF GLUTAMATE BINDING-SITES AND THEIR REGULATION BY CALCIUM IN RAT HIPPOCAMPUS [J].
BAUDRY, M ;
ARST, D ;
OLIVER, M ;
LYNCH, G .
DEVELOPMENTAL BRAIN RESEARCH, 1981, 1 (01) :37-48
[4]   BIOCHEMICAL-CHARACTERIZATION AND LOCALIZATION OF A NON-NORMAL-METHYL-D-ASPARTATE GLUTAMATE RECEPTOR IN RAT-BRAIN [J].
BLACKSTONE, CD ;
MOSS, SJ ;
MARTIN, LJ ;
LEVEY, AI ;
PRICE, DL ;
HUGANIR, RL .
JOURNAL OF NEUROCHEMISTRY, 1992, 58 (03) :1118-1126
[5]   THE EXPRESSION OF EXCITATORY AMINO-ACID BINDING-SITES DURING NEURITOGENESIS IN THE DEVELOPING RAT CEREBELLUM [J].
CAMBRAYDEAKIN, MA ;
FOSTER, AC ;
BURGOYNE, RD .
DEVELOPMENTAL BRAIN RESEARCH, 1990, 54 (02) :265-271
[6]   THE DISTRIBUTION OF GLUTAMATE RECEPTORS IN CULTURED RAT HIPPOCAMPAL-NEURONS - POSTSYNAPTIC CLUSTERING OF AMPA-SELECTIVE SUBUNITS [J].
CRAIG, AM ;
BLACKSTONE, CD ;
HUGANIR, RL ;
BANKER, G .
NEURON, 1993, 10 (06) :1055-1068
[7]   DIFFERENT PROPORTIONS OF N-METHYL-D-ASPARTATE AND NON-N-METHYL-D-ASPARTATE RECEPTOR CURRENTS AT THE MOSSY FIBER GRANULE CELL SYNAPSE OF DEVELOPING RAT CEREBELLUM [J].
DANGELO, E ;
ROSSI, P ;
TAGLIETTI, V .
NEUROSCIENCE, 1993, 53 (01) :121-130
[8]   POSTNATAL-DEVELOPMENT OF THE CHEMOSENSITIVITY OF RAT CEREBELLAR PURKINJE-CELLS TO EXCITATORY AMINO-ACIDS - AN INVITRO STUDY [J].
DUPONT, JL ;
GARDETTE, R ;
CREPEL, F .
DEVELOPMENTAL BRAIN RESEARCH, 1987, 34 (01) :59-68
[9]   DEVELOPMENTAL REGULATION OF MESSENGER-RNAS ENCODING RAT-BRAIN KAINATE AMPA RECEPTORS - A NORTHERN ANALYSIS STUDY [J].
DURAND, GM ;
ZUKIN, RS .
JOURNAL OF NEUROCHEMISTRY, 1993, 61 (06) :2239-2246
[10]   DEVELOPMENTAL EXPRESSION OF EXCITATORY AMINO-ACID RESPONSES IN CEREBELLAR PURKINJE NEURONS IN CULTURE [J].
FRANKLIN, CL ;
GRUOL, DL .
BRAIN RESEARCH, 1991, 543 (02) :219-226