Recombinant adeno-associated virus serotype 2 effectively transduces primary rat brain astrocytes and microglia

被引：8

作者：

Gong, Y

Chen, SZ

Sonntag, CF

Sumners, C

Klein, RL

King, MA

Hughes, JA

Meyer, EM

机构：

[1] Univ Florida, Dept Pharmacol & Therapeut, Coll Med, Gainesville, FL 32610 USA

[2] Univ Florida, Coll Pharm, Dept Pharmaceut, Gainesville, FL 32610 USA

[3] Univ Florida, Coll Pharm, Dept Neurosci, Gainesville, FL 32610 USA

[4] LSUHSC S, Dept Pharmacol & Therapeut, Shreveport, LA 71130 USA

[5] Malcom Randall Vet Adm Med Ctr, Gainesville, FL 32602 USA

来源：

BRAIN RESEARCH PROTOCOLS | 2004年 / 14卷 / 01期

关键词：

recombinant adeno-associated virus 2; green fluorescent protein; primary neurons; astrocytes; microglia;

D O I：

10.1016/j.brainresprot.2004.08.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Recombinant adeno-associated virus-2 (rAAV2) under control of the chicken beta actin promoter/truncated CMV enhancer (CBA) was investigated for its ability to transduce primary cultures of rat brain neurons, microglia and astrocytes. This vector was highly effective in all three cell types in heparin-sensitive manners (astrocytes, microglia and neurons transduced by >98%, 75%, and 95%, respectively). However, astrocytes co-cultured with neurons were not transduced. rAAV2/CBA is an important new method for genetic manipulation of brain cells, though this may be modulated by interactions among cell types. (C) 2004 Elsevier B.V. All rights reserved.

引用

收藏

页码：18 / 24

页数：7

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