How reliable are experimental protein-protein interaction data?

被引：303

作者：

Sprinzak, E ^{[1
]}

Sattath, S ^{[1
]}

Margalit, H ^{[1
]}

机构：

[1] Hebrew Univ Jerusalem, Fac Med, Dept Mol Genet & Biotechnol, IL-91120 Jerusalem, Israel

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2003年 / 327卷 / 05期

基金：

以色列科学基金会;

关键词：

protein-protein interaction; genome-wide analysis; yeast two-hybrid; cellular localization; cellular-role; SACCHAROMYCES-CEREVISIAE; YEAST PROTEOME; COMPLEXES; NETWORK; ORGANIZATION; MAPS;

D O I：

10.1016/S0022-2836(03)00239-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Data of protein-protein interactions provide valuable insight into the molecular networks underlying a living cell. However, their accuracy is often questioned, calling for a rigorous assessment of their reliability. The computation offered here provides an intelligible mean to assess directly the rate of true positives in a data set of experimentally determined interacting protein pairs. We show that the reliability of high-throughput yeast two-hybrid assays is about 50%, and that the size of the yeast interactome is estimated to be 10,000-16,600 interactions. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：919 / 923

页数：5

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