Candida albicans mannan-protein conjugate as vaccine candidate

被引：33

作者：

Bystricky, S

Paulovicová, E

Machová, E

机构：

[1] Slovak Acad Sci, Inst Chem, Bratislava 84238, Slovakia

[2] Natl Inst TB & Resp Dis, Dept Allergy & Clin Immunol, Bratislava 82556, Slovakia

来源：

IMMUNOLOGY LETTERS | 2003年 / 85卷 / 03期

关键词：

conjugate vaccine; Candida albicans; serum IgG; mannan;

D O I：

10.1016/S0165-2478(02)00241-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Mannan-branched polysaccharide with alpha-(1-->6)-linked mannose residues in the linear backbone, is a major virulence and protective factor of yeast Candida albicans. Injected alone does not induce sufficient level of protective antibodies. In this study, we have conjugated mannan to protein carrier by simple one step reaction using 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP) activation reagent. Prepared mannan conjugate is immunogenic in rabbits and reinjection elicited a booster response with significant increase of serum IgG level. Anti-C albicans effectiveness of immune serum is clearly demonstrated. Based on these results, the mannan conjugate synthesized by this scheme can be considered as actual vaccine candidate for clinical evaluation. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：251 / 255

页数：5

共 19 条

[1] IMMUNOGLOBULIN-G ANTIBODIES IN HUMAN VAGINAL SECRETIONS AFTER PARENTERAL VACCINATION [J].