Counting single molecules in sub-nanolitre droplets

被引：53

作者：

Rane, T. D. ^{[1
]}

Puleo, C. M. ^{[1
]}

Liu, K. J. ^{[1
]}

Zhang, Y. ^{[1
]}

Lee, A. P. ^{[2
,3
]}

Wang, T. H. ^{[1
,4
]}

机构：

[1] Johns Hopkins Univ, Dept Biomed Engn, Baltimore, MD 21218 USA

[2] Univ Calif Irvine, Dept Biomed Engn, Irvine, CA USA

[3] Univ Calif Irvine, Dept Mech Aerosp Engn, Irvine, CA 92717 USA

[4] Johns Hopkins Univ, Dept Mech Engn, Baltimore, MD USA

来源：

LAB ON A CHIP | 2010年 / 10卷 / 02期

基金：

美国国家科学基金会;

关键词：

COPY DNA; MICROFLUIDICS; MICRODROPLETS; EXPRESSION; CELLS;

D O I：

10.1039/b917503b

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We demonstrate single biomolecule detection and quantification within sub-nanolitre droplets through application of Cylindrical Illumination Confocal Spectroscopy (CICS) and droplet confinement within a retractable microfluidic constriction.

引用

页码：161 / 164

页数：4

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