A concise method for the preparation of peptide and arginine-rich peptide-conjugated antisense oligonucleotide

被引：27

作者：

Chen, CP

Zhang, LR

Peng, YF

Wang, XB

Wang, SQ

Zhang, LH ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

[2] Peking Univ, Sch Chem & Mol Engn, Beijing 100871, Peoples R China

[3] Beijing Inst Radiat Med, Beijing 100850, Peoples R China

来源：

BIOCONJUGATE CHEMISTRY | 2003年 / 14卷 / 03期

关键词：

D O I：

10.1021/bc034004f

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide-oligonucleotide conjugates were synthesized using two strategies: a mimetic signal peptide-conjugated oligonucleotide was assembled stepwise on CPG by using 2,2-dimethyl-3-hydroxypropionic acid as a linker. To solve the precipitation problem in the coupling reaction caused by the electrostatic interaction of arginine-rich peptides and oligonucleotide, oligonucleotides were absorbed on an anion-exchange resin, and then the on-resin fragment was applied for the conjugation with arginine-rich peptide. The peptide-antisense oligonucleotides showed permeability to the cell membrane of HepG-2 cells.

引用

页码：532 / 538

页数：7

共 30 条

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