Epitope mapping immunodominant regions of the PilA protein of nontypeable Haemophilus influenzae (NTHI) to facilitate the design of two novel chimeric vaccine candidates

被引：51

作者：

Novotny, Laura A. ^{[1
,2
]}

Adams, Leanne D. ^{[1
,2
]}

Kang, D. Richard ^{[2
,3
]}

Wiet, Gregory J. ^{[2
,3
]}

Cai, Xueya ^{[4
,5
]}

Sethi, Sanjay ^{[4
,5
]}

Murphy, Timothy F. ^{[4
,5
]}

Bakaletz, Lauren O. ^{[1
,2
]}

机构：

[1] Nationwide Childrens Hosp, Res Inst, Ctr Microbial Pathogenesis, Columbus, OH 43205 USA

[2] Ohio State Univ, Coll Med, Columbus, OH 43205 USA

[3] Nationwide Childrens Hosp, Dept Otolaryngol, Columbus, OH 43205 USA

[4] SUNY Buffalo, Buffalo, NY 14215 USA

[5] VA Western New York Healthcare Syst, Buffalo, NY 14215 USA

来源：

VACCINE | 2009年 / 28卷 / 01期

关键词：

Chinchilla; Otitis media; COPD; OUTER-MEMBRANE PROTEIN; OTITIS-PRONE CHILDREN; OBSTRUCTIVE PULMONARY-DISEASE; NASOPHARYNGEAL COLONIZATION; CHINCHILLA MODEL; MIDDLE-EAR; IV PILI; MORAXELLA-CATARRHALIS; P5-HOMOLOGOUS ADHESIN; PASSIVE-IMMUNIZATION;

D O I：

10.1016/j.vaccine.2009.08.017

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We designed and tested three PilA-derived vaccine candidates in a chinchilla model of ascending nontypeable Haemophilus influenzae (NTHI)-induced otitis media (OM) Delivery of antiserum directed against each immunogen conferred varying degrees of protection Presentation of a B-cell epitope derived from the OMP P5 adhesin at the N-terminus of recombinant soluble PilA protein (as opposed to the C-terminus), resulted in a protective chimeric immunogen that combined epitopes from two distinct NTHI adhesins (type IV pili and OMP P5) Incorporating protective epitopes derived from two NTHI adhesins/virulence determinants into a single pediatric vaccine candidate to prevent OM has multiple potential inherent advantages (C) 2009 Elsevier Ltd All rights reserved.

引用

页码：279 / 289

页数：11

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