Development and applications of enhanced green fluorescent protein mutants

被引：150

作者：

Stauber, RH ^{[1
]}

Horie, K ^{[1
]}

Carney, P ^{[1
]}

Hudson, EA ^{[1
]}

Tarasova, NI ^{[1
]}

Gaitanaris, GA ^{[1
]}

Pavlakis, GN ^{[1
]}

机构：

[1] NCI, ABL Basic Res Program, FCRDC, Frederick, MD 21702 USA

来源：

BIOTECHNIQUES | 1998年 / 24卷 / 03期

关键词：

D O I：

10.2144/98243rr01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) Florescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.

引用

页码：462 / +

页数：8

共 35 条

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