Development and applications of enhanced green fluorescent protein mutants

被引：150

作者：

Stauber, RH ^{[1
]}

Horie, K ^{[1
]}

Carney, P ^{[1
]}

Hudson, EA ^{[1
]}

Tarasova, NI ^{[1
]}

Gaitanaris, GA ^{[1
]}

Pavlakis, GN ^{[1
]}

机构：

[1] NCI, ABL Basic Res Program, FCRDC, Frederick, MD 21702 USA

来源：

BIOTECHNIQUES | 1998年 / 24卷 / 03期

关键词：

D O I：

10.2144/98243rr01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) Florescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.

引用

页码：462 / +

页数：8

共 35 条

[11] IMPROVED GREEN FLUORESCENCE
HEIM, R
CUBITT, AB
TSIEN, RY
[J]. NATURE, 1995, 373 (6516) : 663 - 664
[12] Engineering green fluorescent protein for improved brightness, longer wavelengths and fluorescence resonance energy transfer
Hein, R
Tsien, RY
[J]. CURRENT BIOLOGY, 1996, 6 (02) : 178 - 182
[13] Visualization of glucocorticoid receptor translocation and intranuclear organization in living cells with a green fluorescent protein chimera
Htun, H
Barsony, J
Renyi, I
Gould, DL
Hager, GL
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (10) : 4845 - 4850
[14] Generation of the replication-competent human immunodeficiency virus type 1 which expresses a jellyfish green fluorescent protein
Lee, AH
Han, JM
Sung, YC
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 233 (01) : 288 - 292
[15] Retroviral transfer and expression of a humanized, red-shifted green fluorescent protein gene into human tumor cells
Levy, JP
Muldoon, RR
Zolotukhin, S
Link, CJ
[J]. NATURE BIOTECHNOLOGY, 1996, 14 (05) : 610 - 614
[16] Tracking and quantitation of retroviral-mediated transfer using a completely humanized, red-shifted green fluorescent protein gene
Muldoon, RR
Levy, JP
Kain, SR
Kitts, PA
Link, CJ
[J]. BIOTECHNIQUES, 1997, 22 (01) : 162 - &
[17] Niedenthal RK, 1996, YEAST, V12, P773, DOI 10.1002/(SICI)1097-0061(19960630)12:8<773::AID-YEA972>3.3.CO
[18] 2-C
[19] QUANTITATIVE IMAGING OF GREEN FLUORESCENT PROTEIN IN CULTURED-CELLS - COMPARISON OF MICROSCOPIC TECHNIQUES, USE IN FUSION PROTEINS AND DETECTION LIMITS
NISWENDER, KD
BLACKMAN, SM
ROHDE, L
MAGNUSON, MA
PISTON, DW
[J]. JOURNAL OF MICROSCOPY, 1995, 180 : 109 - 116
[20] The structural basis for spectral variations in green fluorescent protein
Palm, GJ
Zdanov, A
Gaitanaris, GA
Stauber, R
Pavlakis, GN
Wlodawer, A
[J]. NATURE STRUCTURAL BIOLOGY, 1997, 4 (05) : 361 - 365

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