Label-free electrochemical detection of DNA in blood serum via target-induced resolution of an electrode-bound DNA pseudoknot

被引：228

作者：

Xiao, Yi

Qu, Xiaogang

Plaxco, Kevin W. ^{[1
]}

Heeger, Alan J.

机构：

[1] Chinese Acad Sci, Changchun Inst Appl Chem, Div Biol Inorgan Chem, Key Lab Rare Earth Chem & Phys, Changchun 130022, Jilin, Peoples R China

[2] Univ Calif Santa Barbara, Dept Phys, Santa Barbara, CA 93106 USA

[3] Univ Calif Santa Barbara, Inst Polymers & Organ Solids, Dept Chem & Biochem, Santa Barbara, CA 93106 USA

[4] Univ Calif Santa Barbara, Program BioMol Sci & Engn, Santa Barbara, CA 93106 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 39期

关键词：

D O I：

10.1021/ja074218y

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have demonstrated a fully covalent, signal-on E-DNA architecture based on the target-induced resolution of a DNA pseudokont. In the absence of target, the electrode-bound DNA probe adopts a pseudoknot conformation that segregates an attached methylene blue (MB) from the electrode. Upon target binding, the pseudoknot is resolved, leading to the formation of a single-stranded DNA element that supports electron transfer from the methylene blue to the electrode.

引用

页码：11896 / +

页数：3

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