DNA-PEG-DNA triblock macromolecules for reagentless DNA detection

被引：158

作者：

Immoos, CE

Lee, SJ

Grinstaff, MW ^{[1
]}

机构：

[1] Boston Univ, Metcalf Ctr Sci & Engn, Dept Biomed Engn, Boston, MA 02215 USA

[2] Boston Univ, Metcalf Ctr Sci & Engn, Dept Chem, Boston, MA 02215 USA

[3] USA, Res Off, Res Triangle Pk, NC 27709 USA

[4] Calif Polytech State Univ San Luis Obispo, Dept Chem & Biochem, San Luis Obispo, CA 93407 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 35期

关键词：

D O I：

10.1021/ja046634d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The sandwich assay is the most common design for electrochemical DNA sensors. This assay consists of three individual DNA components: an immobilized capture strand, a target strand, and a probe strand containing a redox-active reporter group. We report a simplified DNA assay where two strands of ssDNA, the capture and probe strands, are linked together via a flexible poly(ethylene glycol) (PEG) spacer forming an ABA triblock macromolecule. We have developed an electrochemical assay where the detection signal arises as a consequence of a large structural change induced upon hybridization with target DNA. In this system, the DNA-PEG-DNA macromolecule folds or wraps around the target DNA, bringing the ferrocene probe in close proximity to the electrode, affording an electrochemical response. Copyright © 2003 American Chemical Society.

引用

页码：10814 / 10815

页数：2

共 24 条

[1] 3′-ferrocene-labeled oligonucleotide chains end-tethered to gold electrode surfaces:: Novel model systems for exploring flexibility of short DNA using cyclic voltammetry [J].