A common SCN5A polymorphism modulates the biophysical effects of an SCN5A mutation

被引:166
作者
Viswanathan, PC
Benson, DW
Balser, JR
机构
[1] Vanderbilt Univ, Sch Med, Dept Anesthesiol, Nashville, TN 37232 USA
[2] Childrens Hosp, Med Ctr, Cincinnati, OH 45229 USA
[3] Vanderbilt Univ, Sch Med, Dept Pharmacol, Nashville, TN 37212 USA
关键词
D O I
10.1172/JCI200316879
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Our understanding of the genetic basis of disease has expanded with the identification of rare DNA sequence variations ("mutations") that evoke inherited syndromes such as cystic fibrosis, congenital epilepsy, and cardiac arrhythmias. Common sequence variants ("polymorphisms") have also been implicated as risk factors in multiple diseases. Mutations in SCN5A, the cardiac Na+ channel gene, that cause a reduction in Na+ current may evoke severe, life-threatening disturbances in cardiac rhythm (i.e., Brugada. syndrome), isolated cardiac conduction disease, or combinations of these disorders. Conduction disease is manifest clinically as heart rate slowing (bradycardia), syncope, or "lightheadedness". Recent electrophysiologic studies reveal that mutations in particular families exhibiting cardiac conduction disease cause marked effects on several competing voltage-dependent gating processes, but nonetheless cause a mild "net" reduction in Na+ current. Here we show that a common SCN5A polymorphism (H558R) in the Na+ channel I-II interdomain cytoplasmic linker, present in 20% of the population, can mitigate the in vitro effects of a nearby mutation (TS 12I) on Na+ channel function. The mutation and the polymorphism were both found in the same allele of a child with isolated conduction disease, suggesting a direct functional association between a polymorphism and a mutation in the same gene.
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收藏
页码:341 / 346
页数:6
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