Determinants of surfactant function in acute lung injury and early recovery

被引:19
作者
Mora, R
Arold, S
Marzan, Y
Suki, B
Ingenito, EP
机构
[1] Brigham & Womens Hosp, Div Pulm & Crit Care Med, Boston, MA 02115 USA
[2] Beth Israel Deaconess Med Ctr, Div Pulm & Crit Care Med, Boston, MA 02115 USA
[3] Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA
关键词
acute respiratory distress syndrome; lipopolysaccharide; apoprotein; pulsating bubble surfactometry; optimal ventilator waveform;
D O I
10.1152/ajplung.2000.279.2.L342
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Relationships between lung function and surfactant function and composition were examined during the evolution of acute lung injury in guinea pigs. Lung mechanics and gas exchange were assessed 12, 24, or 48 h after exposure to nebulized lipopolysaccharide (LPS). Bronchoalveolar lavage (BAL) fluid was processed for phospholipid and protein contents and surfactant protein (SP) A and SP-B levels; surfactant function was measured by pulsating bubble surfactometry. Lung elastance, tissue resistance, and arterial-alveolar gradient were moderately elevated by 12 h after LPS exposure and continued to increase over the first 24 h but began to recover between 24 and 48 h. Similarly, the absolute amount of 30,000 g pelleted SP-A and SP-B, the phospholipid content of BAL fluid, and surfactant function declined over the first 24 h after exposure, with recovery between 24 and 48 h. BAL fluid total protein content increased steadily over the first 48 h after LPS nebulization. In this model of acute lung injury, the intra-alveolar repletion of surfactant components in early recovery led to improved surfactant function despite the presence of potentially inhibitory plasma proteins.
引用
收藏
页码:L342 / L349
页数:8
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