Directed evolution of ribosomal protein S1 for enhanced translational efficiency of high GC Rhodopseudomonas palustris DNA in Escherichia coli

被引：14

作者：

Bernstein, Jeffrey R.

Bulter, Thomas

Shen, Claire R.

Liao, James C.

机构：

[1] Univ Calif Los Angeles, Dept Chem & Biomol Engn, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Biomed Engn & Interdepartamental Program, Los Angeles, CA 90095 USA

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 2007年 / 282卷 / 26期

关键词：

D O I：

10.1074/jbc.M701395200

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The expression of foreign DNA in Escherichia coli is important in biotechnological applications. However, the translation of genes from GC-rich organisms is inefficient in E. coli. To overcome this problem, we applied directed evolution to E. coli ribosomal protein S1. Two selected mutants enabled 12- and 8-fold higher expression levels from GC-rich DNA targets. General improvements in translation efficiency over a range of genes from Rhodopseudomonas palustris and E. coli was achieved using an S1 mutant selected against multiple genes from R. palustris. This method opens new opportunities for the expression of GC-rich genes in E. coli.

引用

页码：18929 / 18936

页数：8

共 43 条

[1]

ARVIDSON DN, 1991, BIOTECHNIQUES, V11, P733

[2] The last RNA-binding repeat of the Escherichia coli ribosomal protein S1 is specifically involved in autogenous control [J].