An efficient method for solving RNA structures: MAD phasing by replacing magnesium with zinc

被引：15

作者：

Ennifar, E ^{[1
]}

Walter, P ^{[1
]}

Dumas, P ^{[1
]}

机构：

[1] Inst Biol Mol & Cellulaire, CNRS, UPR 9002, F-67084 Strasbourg, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2001年 / 57卷

关键词：

D O I：

10.1107/S0907444900017558

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The structure of a 46-nucleotide RNA complex has been successfully solved using multi-wavelength anomalous dispersion (MAD) at the zinc K edge. Taking advantage of the eight magnesium-binding sites, it has been shown that for five of them magnesium could be replaced by zinc. This resulted in an excellent 2.0 Angstrom MAD electron-density map. Zinc, in common with some other transition metals, is able to replace magnesium in RNA structures, but zinc has the advantage of its K edge being ideally located at 1.284 Angstrom. As most RNA molecules contain magnesium-binding sites, it is suggested that this method could be a valuable alternative to the use of bromo derivatives of bases, which is limited to chemically synthesizable and thus rather short RNA sequences.

引用

页码：330 / 332

页数：3

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