Genetically Encoded Alkenes in Yeast

被引：59

作者：

Ai, Hui-wang ^{[1
]}

Shen, Weijun ^{[1
]}

Brustad, Eric ^{[1
]}

Schultz, Peter G. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 05期

关键词：

nonnatural amino acids; olefin metathesis; protein modification; TRANSFER-RNA SYNTHETASE; SELECTIVE PROTEIN MODIFICATION; OLEFIN METATHESIS; ESCHERICHIA-COLI; IN-VIVO; EFFICIENT INCORPORATION; PHOTOCLICK CHEMISTRY; LIGATION; FUNCTIONALIZATION; DEHYDROALANINE;

D O I：

10.1002/anie.200905590

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Olefin-containing amino acids have been genetically introduced into proteins in Saccharomyces cerevisiae by orthogonal tRNA/aminoacyl-tRNA synthetase pairs. These nonnatural amino acids can be used for selective protein modification through olefin metathesis reactions. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

收藏

页码：935 / 937

页数：3

相关论文

共 38 条

[1]

Ai H.-W., 2007, BIOTECHNOL FOCUS, V10, P16

[2]

[Anonymous], 2009, ANGEW CHEM

[3] Reactivation of the p53 tumor suppressor pathway by a stapled p53 peptide [J].

Bernal, Federico ;

Tyler, Andrew F. ;

Korsmeyer, Stanley J. ;

Walensky, Loren D. ;

Verdine, Gregory L. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2007, 129 (09) :2456-+

[4] Facile conversion of cysteine and alkyl cysteines to dehydroalanine on protein surfaces: Versatile and switchable access to functionalized proteins [J].

Bernardes, Goncalo J. L. ;

Chalker, Justin M. ;

Errey, James C. ;

Davis, Benjamin G. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (15) :5052-+

[5] Olefin metathesis for chemical biology [J].

Binder, Joseph B. ;

Raines, Ronald T. .

CURRENT OPINION IN CHEMICAL BIOLOGY, 2008, 12 (06) :767-773

[6] Tetrazine ligation: Fast bioconjugation based on inverse-electron-demand Diels-Alder reactivity [J].

Blackman, Melissa L. ;

Royzen, Maksim ;

Fox, Joseph M. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2008, 130 (41) :13518-+

[7] Metathesis in peptides and peptidomimetics [J].

Brik, Ashraf .

ADVANCED SYNTHESIS & CATALYSIS, 2008, 350 (11-12) :1661-1675

[8] A promiscuous aminoacyl-tRNA synthetase that incorporates cysteine, methionine, and alanine homologs into proteins [J].

Brustad, Eric ;

Bushey, Mark L. ;

Brock, Ansgar ;

Chittuluru, Johnathan ;

Schultz, Peter G. .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2008, 18 (22) :6004-6006

[9] Enabling olefin metathesis on proteins: chemical methods for installation of S-allyl cysteine [J].

Chalker, Justin M. ;

Lin, Yuya A. ;

Boutureira, Omar ;

Davis, Benjamin G. .

CHEMICAL COMMUNICATIONS, 2009, (25) :3714-3716

[10] CP1 domain in Escherichia coli leucyl-tRNA synthetase is crucial for its editing function [J].

Chen, JF ;

Guo, NN ;

Li, T ;

Wang, ED ;

Wang, YL .

BIOCHEMISTRY, 2000, 39 (22) :6726-6731

← 1 2 3 4 →