Genomic homology of the domestic ferret with cats and humans

被引:40
作者
Cavagna, P
Menotti, A
Stanyon, R
机构
[1] NCI, Mol Cytogenet Sect, Frederick, MD 21702 USA
[2] Univ Genoa, Dept Expt Biol, I-16132 Genoa, Italy
[3] Univ Genoa, Dept Anthropol Sci, Basic Res Lab, I-16132 Genoa, Italy
关键词
D O I
10.1007/s003350010172
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We used chromosome paints from both the domestic cat and humans to directly establish chromosomal homology between the genome of these species and the domestic ferret. The chromosome painting data indicate that the ferret has a highly conserved karyotype closer to the ancestral carnivore karyotype than that of the cat. The cat chromosome paints revealed 22 homologous autosomal regions in the ferret genome: 16 ferret chromosomes were hybridized by a single cat paint, while 3 ferret chromosomes were hybridized by two cat paints. In situ hybridization combined with banding showed that ferret Chromosome (Chr) 1 = cat A2p/C2, Chr 2 = F2/C1q, and Chr 3 = A2q/D2. Five ferret chromosomes are homologous to single arms of cat chromosomes: ferret 4 = A1q, 5 = Big, 6 = Clp, 10 = Alp, and 12 = B1p. The human chromosome paints revealed 32 + XY homologous regions in the ferret genome: 9 ferret chromosomes were each hybridized by a single human paint, 7 by two paints, 3 by three paints. The 10 ferret chromosomes hybridized by multiple human paints produced the following associations: ferret 1 = human 19/3/21, 2 = 8q/2q, 3 = 10/7,5 = 8/4, 8 = 15/14, 9 = 10/12/22, 11 = 20/2, 12 = 8/4, 14 = 12/22/18, 18 = 19/16. We present an index of genomic diversity, Z, based on the relative number of conserved whole chromosome and chromosome segments as a preliminary statistic for rapid comparison between species. The index of diversity between human-ferret (Z = 0.812) is slightly less than human-cat (Z = 0.843). The homology data presented here allow us to transfer gene mapping data from both cats and humans to the ferret.
引用
收藏
页码:866 / 870
页数:5
相关论文
共 37 条
[1]  
Alder JD, 1996, AM J GASTROENTEROL, V91, P2347
[2]   Homologies between human and dolphin chromosomes detected by heterologous chromosome painting [J].
Bielec, PE ;
Gallagher, DS ;
Womack, JE ;
Busbee, DL .
CYTOGENETICS AND CELL GENETICS, 1998, 81 (01) :18-25
[3]  
Carson JL, 1999, ANAT REC, V256, P242, DOI 10.1002/(SICI)1097-0185(19991101)256:3<242::AID-AR4>3.0.CO
[4]  
2-C
[5]  
Chapman B, 2000, J NEUROSCI, V20, P1922
[6]   Comparative analysis of the cattle and human genomes: Detection of ZOO-FISH and gene mapping-based chromosomal homologies [J].
Chowdhary, BP ;
Fronicke, L ;
Gustavsson, I ;
Scherthan, H .
MAMMALIAN GENOME, 1996, 7 (04) :297-302
[7]   Systematics of mustelid-like carnivores [J].
Dragoo, JW ;
Honeycutt, RL .
JOURNAL OF MAMMALOGY, 1997, 78 (02) :426-443
[8]   THE ANCESTRAL KARYOTYPE OF CARNIVORA - COMPARISON WITH THAT OF PLATYRRHINE MONKEYS [J].
DUTRILLAUX, B ;
COUTURIER, J .
CYTOGENETICS AND CELL GENETICS, 1983, 35 (03) :200-208
[9]   Clusters of lymphoma in ferrets [J].
Erdman, SE ;
Kanki, PJ ;
Moore, FM ;
Brown, SA ;
Kawasaki, TA ;
Mikule, KW ;
Travers, KU ;
Badylak, SF ;
Fox, JG .
CANCER INVESTIGATION, 1996, 14 (03) :225-230
[10]   Reciprocal chromosome painting shows that the great difference in diploid number between human and African green monkey is mostly due to non-Robertsonian fissions [J].
Finelli, P ;
Stanyon, R ;
Plesker, R ;
Ferguson-Smith, MA ;
O'Brien, PCM ;
Wienberg, J .
MAMMALIAN GENOME, 1999, 10 (07) :713-718