Regulation of telomere length by checkpoint genes in Schizosaccharomyces pombe

被引:60
作者
Dahlén, M [1 ]
Olsson, T [1 ]
Kanter-Smoler, G [1 ]
Ramne, A [1 ]
Sunnerhagen, P [1 ]
机构
[1] Gothenburg Univ, Lundberg Lab, Dept Biol Mol, S-40530 Gothenburg, Sweden
关键词
D O I
10.1091/mbc.9.3.611
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We have studied telomere length in Schizosaccharomyces pombe strains carrying mutations affecting cell cycle checkpoints, DNA repair, and regulation of the Cdc2 protein kinase. Telomere shortening was found in rad1, rad3, rad17, and rad26 mutants. Telomere lengths in previously characterized rad1 mutants paralleled the replication checkpoint proficiency of those mutants. In contrast, rad9, chk1, hus1, and cds1 mutants had intact telomeres. No difference in telomere length was seen in mutants affected in the regulation of Cdc2, whereas some of the DNA repair mutants examined had slightly longer telomeres than did the wild type. Overexpression of the rad1(+) gene caused telomeres to elongate slightly. The kinetics of telomere shortening was monitored by following telomere length after disruption of the rad1(+) gene; the rate was similar to 1 nucleotide per generation. Wild-type telomere length could be restored by reintroduction of the wild-type rad1(+) gene. Expression of the Saccharomyces cerevisiae RCK1 protein kinase gene, which suppresses the radiation and hydroxyurea sensitivity of St, pombe checkpoint mutants, was able to attenuate telomere shortening in rad1 mutant cells and to increase telomere length in a wild-type background. The functional effects of telomere shortening in rad1 mutants were assayed by measuring loss of a linear and a circular minichromosome. A minor increase in loss rate was seen with the linear minichromosome, and an even smaller difference compared with wild-type was detected with the circular plasmid.
引用
收藏
页码:611 / 621
页数:11
相关论文
共 60 条
  • [1] DNA-REPAIR MUTANTS DEFINING G2 CHECKPOINT PATHWAYS IN SCHIZOSACCHAROMYCES-POMBE
    ALKHODAIRY, F
    CARR, AM
    [J]. EMBO JOURNAL, 1992, 11 (04) : 1343 - 1350
  • [2] IDENTIFICATION AND CHARACTERIZATION OF NEW ELEMENTS INVOLVED IN CHECKPOINT AND FEEDBACK CONTROLS IN FISSION YEAST
    ALKHODAIRY, F
    FOTOU, E
    SHELDRICK, KS
    GRIFFITHS, DJF
    LEHMANN, AR
    CARR, AM
    [J]. MOLECULAR BIOLOGY OF THE CELL, 1994, 5 (02) : 147 - 160
  • [3] MUTATIONS DEREPRESSING SILENT CENTROMERIC DOMAINS IN FISSION YEAST DISRUPT CHROMOSOME SEGREGATION
    ALLSHIRE, RC
    NIMMO, ER
    EKWALL, K
    JAVERZAT, JP
    CRANSTON, G
    [J]. GENES & DEVELOPMENT, 1995, 9 (02) : 218 - 233
  • [4] CLONING AND CHARACTERIZATION OF RAD21 AN ESSENTIAL GENE OF SCHIZOSACCHAROMYCES-POMBE INVOLVED IN DNA DOUBLE-STRAND-BREAK REPAIR
    BIRKENBIHL, RP
    SUBRAMANI, S
    [J]. NUCLEIC ACIDS RESEARCH, 1992, 20 (24) : 6605 - 6611
  • [5] Identification of a Saccharomyces cerevisiae Ku80 homologue: Roles in DNA double strand break rejoining and in telomeric maintenance
    Boulton, SJ
    Jackson, SP
    [J]. NUCLEIC ACIDS RESEARCH, 1996, 24 (23) : 4639 - 4648
  • [6] THE CELLULAR-RESPONSES TO DNA-DAMAGE
    CARR, AM
    HOEKSTRA, MF
    [J]. TRENDS IN CELL BIOLOGY, 1995, 5 (01) : 32 - 40
  • [7] THE RAD16 GENE OF SCHIZOSACCHAROMYCES-POMBE - A HOMOLOG OF RAD1 GENE OF SACCHAROMYCES-CEREVISIAE
    CARR, AM
    SCHMIDT, H
    KIRCHHOFF, S
    MURIEL, WJ
    SHELDRICK, KS
    GRIFFITHS, DJ
    BASMACIOGLU, CN
    SUBRAMANI, S
    CLEGG, M
    NASIM, A
    LEHMANN, AR
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (03) : 2029 - 2040
  • [8] Regulation of telomere length and function by a Myb-domain protein in fission yeast
    Cooper, JP
    Nimmo, ER
    Allshire, RC
    Cech, TR
    [J]. NATURE, 1997, 385 (6618) : 744 - 747
  • [9] 2 NOVEL DEDUCED SERINE/THREONINE PROTEIN-KINASES FROM SACCHAROMYCES-CEREVISIAE
    DAHLKVIST, A
    SUNNERHAGEN, P
    [J]. GENE, 1994, 139 (01) : 27 - 33
  • [10] THE RCK1 AND RCK2 PROTEIN-KINASE GENES FROM SACCHAROMYCES-CEREVISIAE SUPPRESS CELL-CYCLE CHECKPOINT MUTATIONS IN SCHIZOSACCHAROMYCES-POMBE
    DAHLKVIST, A
    KANTERSMOLER, G
    SUNNERHAGEN, P
    [J]. MOLECULAR AND GENERAL GENETICS, 1995, 246 (03): : 316 - 326