Chitosan produces potent neuroprotection and physiological recovery following traumatic spinal cord injury

被引:99
作者
Cho, Youngnam [1 ]
Shi, Riyi [1 ,2 ]
Borgens, Richard B. [1 ,2 ]
机构
[1] Purdue Univ, Sch Vet Med, Ctr Paralysis Res, W Lafayette, IN 47907 USA
[2] Purdue Univ, Weldon Sch Biomed Engn, W Lafayette, IN 47907 USA
关键词
chitosan; nanoparticle; nerve tissue engineering; membrane; self assembly; ATOMIC-FORCE MICROSCOPY; POLYETHYLENE-GLYCOL; OXIDATIVE STRESS; SURFACTANT POLOXAMER-188; PHOSPHOLIPID-BILAYER; MOLECULAR REPAIR; BRAIN-INJURY; PC12; CELLS; IN-VITRO; MEMBRANES;
D O I
10.1242/jeb.035162
中图分类号
Q [生物科学];
学科分类号
090105 [作物生产系统与生态工程];
摘要
Chitosan, a non-toxic biodegradable polycationic polymer with low immunogenicity, has been extensively investigated in various biomedical applications. In this work, chitosan has been demonstrated to seal compromised nerve cell membranes thus serving as a potent neuroprotector following acute spinal cord trauma. Topical application of chitosan after complete transection or compression of the guinea pig spinal cord facilitated sealing of neuronal membranes in ex vivo tests, and restored the conduction of nerve impulses through the length of spinal cords in vivo, using somatosensory evoked potential recordings. Moreover, chitosan preferentially targeted damaged tissues, served as a suppressor of reactive oxygen species ( free radical) generation, and the resultant lipid peroxidation of membranes, as shown in ex vivo spinal cord samples. These findings suggest a novel medical approach to reduce the catastrophic loss of behavior after acute spinal cord and brain injury.
引用
收藏
页码:1513 / 1520
页数:8
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