Screening and characterization of human single-chain Fv antibody against β-amyloid peptide 40

被引：11

作者：

Cai, J

Wang, SZ ^{[1
]}

Zhong, YW

Cheng, J

Ji, ZJ

Sheng, SL

Zhang, C

机构：

[1] Beijing Union Med Coll Hosp, Dept Nucl Med, Beijing 100730, Peoples R China

[2] Chinese Acad Med Sci, Beijing 100730, Peoples R China

[3] 302 Hosp PLA, Inst Infect Dis, Gene Therapy Res Ctr, Beijing 100039, Peoples R China

[4] Capital Univ Med Sci, Xuanwu Hosp, Beijing Res Lab Brain Aging, Beijing 100053, Peoples R China

来源：

NEUROREPORT | 2003年 / 14卷 / 02期

关键词：

Alzheimer's disease; amyloid-beta pepticle; phage display; single-chain antibody;

D O I：

10.1097/00001756-200302100-00022

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

Expression of single-chain variable fragment antibody on the surface of filamentous bacteriophage is widely used to make antibodies with pre-defined specificities. Using direct selection on solid phase-bound amyloid-beta 40 peptide, wich is a main pathogenic feature of Alzheimer's disease, we obtained single-chain Fv antibody with specific binding activity. The binding epitope of the antibody is located between amino acids 1 and 16 of the peptide antigen. DNA sequencing showed that the gene coding for the single chain Fv antibody consists of 768 bp, and the complementarity-determining regions were deduced.

引用

页码：265 / 268

页数：4

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