Enhancement of proteolysis through the silica-gel-derived microfluidic reactor

被引：19

作者：

Liu, Yun

Qu, Haiyun

Xue, Yan

Wu, Zhonglin

Yang, Pengyuan

Liu, Baohong ^{[1
]}

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[2] Fudan Univ, Inst Biomed Sci, Shanghai 200433, Peoples R China

来源：

PROTEOMICS | 2007年 / 7卷 / 09期

关键词：

mass; spectra microfluidics; proteolysis;

D O I：

10.1002/pmic.200600896

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An on-chip enzymatic reactor providing rapid protein digestion is presented. Trypsin-embedding stationary phase within the microchannel has been prepared by the sol-gel method. Such a microfluidic reactor has been used for low-level protein digestion at 16 fmol per analysis. The analytical potential of the microreactor combined with the strong cation exchange and RPLC ESI-MS/MS for the identification of real samples from the cytoplasma of the human liver tissue has been demonstrated.

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收藏

页码：1373 / 1378

页数：6

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