Proteins Required for Lipopolysaccharide Assembly in Escherichia coli Form a Transenvelope Complex

被引：123

作者：

Chng, Shu-Sin

Gronenberg, Luisa S.

Kahne, Daniel ^{[1
]}

机构：

[1] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

BIOCHEMISTRY | 2010年 / 49卷 / 22期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

OUTER-MEMBRANE BIOGENESIS; SALMONELLA-TYPHIMURIUM; INNER-MEMBRANE; CELL-ENVELOPE; LIPID-A; TRANSPORT; IDENTIFICATION; LPTA; ADHESION; EXPORT;

D O I：

10.1021/bi100493e

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The viability of Gram-negative organisms is dependent on the proper placement of lipopolysaccharide (LPS) in the outer leaflet of its outer membrane. LPS is synthesized inside the cell and transported to the surface by seven essential lipopolysaccharide transport (Lpt) proteins. How these proteins cooperate to transport LPS is unknown. We show that these Lpt proteins can be found in a membrane fraction that contains inner and outer membranes and that they copurify. This constitutes the first evidence that the Lpt proteins form a transenvelope complex. We suggest that this protein bridge provides a route for LPS transport across the cell envelope.

引用

收藏

页码：4565 / 4567

页数：3

相关论文

共 29 条

[1] AREAS OF ADHESION BETWEEN WALL AND MEMBRANE OF ESCHERICHIA COLI [J].

BAYER, ME .

JOURNAL OF GENERAL MICROBIOLOGY, 1968, 53 :395-&

[2] ZONES OF MEMBRANE ADHESION IN THE CRYOFIXED ENVELOPE OF ESCHERICHIA-COLI [J].

BAYER, ME .

JOURNAL OF STRUCTURAL BIOLOGY, 1991, 107 (03) :268-280

[3] Biogenesis of the gram-negative bacterial outer membrane [J].

Bos, Martine P. ;

Robert, Viviane ;

Tommassen, Jan .

ANNUAL REVIEW OF MICROBIOLOGY, 2007, 61 :191-214

[4] Identification of an outer membrane protein required for the transport of lipopolysaccharide to the bacterial cell surface [J].

Bos, MP ;

Tefsen, B ;

Geurtsen, J ;

Tommassen, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (25) :9417-9422

[5] Imp/OstA is required for cell envelope biogenesis in Escherichia coli [J].

Braun, M ;

Silhavy, TJ .

MOLECULAR MICROBIOLOGY, 2002, 45 (05) :1289-1302

[6] Characterization of the two-protein complex in Escherichia coli responsible for lipopolysaccharide assembly at the outer membrane [J].

Chng, Shu-Sin ;

Ruiz, Natividad ;

Chimalakonda, Gitanjali ;

Silhavy, Thomas J. ;

Kahne, Daniel .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (12) :5363-5368

[7] The Pfam protein families database [J].

Finn, Robert D. ;

Tate, John ;

Mistry, Jaina ;

Coggill, Penny C. ;

Sammut, Stephen John ;

Hotz, Hans-Rudolf ;

Ceric, Goran ;

Forslund, Kristoffer ;

Eddy, Sean R. ;

Sonnhammer, Erik L. L. ;

Bateman, Alex .

NUCLEIC ACIDS RESEARCH, 2008, 36 :D281-D288

[8] Development of an Activity Assay for Discovery of Inhibitors of Lipopolysaccharide Transport [J].

Gronenberg, Luisa S. ;

Kahne, Daniel .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (08) :2518-+

[9] Bioinformatic analyses of Gram-negative bacterial OstA outer membrane assembly homologues [J].

Hu, Kuang-Yu ;

Saier, Milton H., Jr. .

CURRENT GENOMICS, 2006, 7 (07) :447-461

[10]

ISHIDATE K, 1986, J BIOL CHEM, V261, P428