Improved lipid-mediated gene transfer into primary cultures of hippocampal neurons

被引：37

作者：

Kaech, S ^{[1
]}

Kim, JB ^{[1
]}

Cariola, M ^{[1
]}

Ralston, E ^{[1
]}

机构：

[1] NINCDS,NEUROBIOL LAB,NATL INST HLTH,BETHESDA,MD 20892

来源：

MOLECULAR BRAIN RESEARCH | 1996年 / 35卷 / 1-2期

关键词：

transfection; neuronal culture; hippocampus; liposomes; beta-galactosidase;

D O I：

10.1016/0169-328X(95)00238-N

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have examined lipids as transfection agents to introduce recombinant plasmids into primary cultures of rat hippocampal neurons. By modifying the protocol for transfection mediated by the commercial reagent DOTAP, we were able to achieve a transfection efficiency of about 3%. Expression of various transfected gene products was sustained for several weeks in culture, the neurons developed normally and the transfected gene products were targeted to the appropriate subcellular compartment.

引用

页码：344 / 348

页数：5

共 22 条

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