Basic neural transplantation techniques. I. Dissociated cell suspension grafts of embryonic ventral mesencephalon in the adult rat brain

被引：46

作者：

Dunnett, SB

Bjorklund, A

机构：

[1] MRC, Cambridge Ctr Brain Repair, Cambridge CB2 2PY, England

[2] Univ Cambridge, Dept Expt Psychol, Cambridge CB2 2PY, England

[3] Lund Univ, Dept Med Cell Res, S-22362 Lund, Sweden

来源：

BRAIN RESEARCH PROTOCOLS | 1997年 / 1卷 / 01期

关键词：

neural transplantation; embryonic development; dopamine-rich graft; nigral graft; ventral mesencephalic graft; animal model of parkinsonism;

D O I：

10.1016/S1385-299X(96)00015-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Lesions and grafts in the nigrostriatal dopamine system in rats is widely used as a model of degeneration and regeneration in the CNS, and the development for alternative strategies for treatment in Parkinson's disease. The methods of preparing a dissociated cell suspension of embryonic rat substantia nigra and its transplantation into the brain of adult rats are described, This is the first of a series of methodological reports on the basic methodology and refinements of neural transplantation techniques in the mammalian central nervous system.

引用

页码：91 / 99

页数：9

共 33 条

[31] AUTOREGULATION OF DOPAMINE RELEASE AND METABOLISM BY INTRASTRIATAL NIGRAL GRAFTS AS REVEALED BY INTRACEREBRAL DIALYSIS [J].