Identification of sex pheromone of adzuki bean borer, Ostrinia scapulalis

By means of gas chromatography with electroantennographic detection (GC-EAD), gas chromatography-mass spectrometry (GC-MS), and a series of bioassays, (Z)-11-tetradecenyl acetate (Z11-14:OAc) and (E)-11-tetradecenyl acetate (E11-14:OAc) at a ratio of 100:3 were identified as the female sex pheromone of the adzuki bean borer, Ostrinia scapulalis. The average amounts of Z11-14 : OAc and E11-14 : OAc in a single sex pheromone gland were 6.6 +/- 2.4 ng and 0.2 +/- 0.1 ng, respectively. In a wind-tunnel bioassay, the binary blend of Z11- and E11-14:OAc elicited almost the same male behavioral responses as did virgin females and sex pheromone gland extract. In field trapping experiments, rubber septa impregnated with the binary blend (50 mu g/septum) attracted more males than virgin females. The sex pheromone of O. scapulalis thus turned out to be similar to that of the Z-type European corn borer, O. nubilalis, in both components and their ratio.