A novel defensin encoded by a mungbean cDNA exhibits insecticidal activity against bruchid

被引：82

作者：

Chen, KC

Lin, CY

Kuan, CC

Sung, HY

Chen, CS ^{[1
]}

机构：

[1] Natl Taiwan Univ, Grad Inst Agr Chem, Taipei 106, Taiwan

[2] Acad Sinica, Inst Bot, Taipei 115, Taiwan

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2002年 / 50卷 / 25期

关键词：

defensin; bruchid resistance; mungbean; in vitro translation;

D O I：

10.1021/jf020527q

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

A cDNA encoding a small cysteine-rich protein designated VrCRP was isolated from a bruchid-resistant mungbean. VrCRP encodes a protein of 73 amino acids containing a 27 amino acid signal peptide and 8 cysteines. On the basis of the amino acid sequence similarity and conserved residues, it is suggested that VrCRP is a member of the plant defensin family. VrCRP protein was obtained by overexpression of VrCRP with a truncated signal peptide in an IMPACT system. Artificial seeds containing 0.2% (w/w) of the purified VrCRP-TSP were lethal to larvae of the bruchid Callosobruchus chinensis. VrCRP is apparently the first reported plant defensin exhibiting in vitro insecticidal activity against C, chinensis.

引用

页码：7258 / 7263

页数：6

共 39 条

[1] A NEW FAMILY OF SMALL (5 KDA) PROTEIN INHIBITORS OF INSECT ALPHA-AMYLASES FROM SEEDS OR SORGHUM (SORGHUM-BICOLOR (L) MOENCH) HAVE SEQUENCE HOMOLOGIES WITH WHEAT GAMMA-PUROTHIONINS [J].