Electrostatic properties and macroscopic electrodiffusion in OmpF porin and mutants

被引:41
作者
Aguilella-Arzo, Marcel [1 ]
Garcia-Celma, Juan J. [1 ]
Cervera, Javier [1 ]
Alcaraz, Antonio [1 ]
Aguilella, Vicente M. [1 ]
机构
[1] Univ Jaume 1, Dept Expt Sci, Biophys Unit, E-12080 Castellon de La Plana, Spain
关键词
electrodiffusion; OmpF; bacterial porin; channel selectivity;
D O I
10.1016/j.bioelechem.2006.04.005
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The bacterial porin OmpF found in the outer membrane of E. coli is a wide channel, characterized by its poor selectivity and almost no ion specificity. It has an asymmetric structure, with relatively large entrances and a narrow constriction. By applying continuum electrostatic methods we determine the ionization states of titratable amino acid residues in the protein and calculate self-consistently the electric potential 3-D distribution within the channel. The average electrostatic properties are then represented by an effective fixed charge distribution along the pore which is the input for a macroscopic electrodiffusion model. The theoretical predictions agree with measurements performed under different salt gradients and pH. The sensitivity of reversal potential and conductance to the direction of the salt gradient and the solution pH is captured by the model. The theory is also able to explain the influence of the lipid membrane charge. The same methodology is satisfactorily applied to some OmpF mutants involving slight structural changes but a large number of net charges. The correlation found between atomic structure and ionic selectivity shows that the transport characteristics of wide channels like OmpF and its mutants are mainly regulated by the collective action of a large number of residues, rather than by the specific interactions of residues at particular locations. (C) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:320 / 327
页数:8
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