Retention of biological activity and near-infrared absorbance upon adsorption of horseradish peroxidase on single-walled carbon nanotubes

被引：30

作者：

Palwai, Naveen R.

Martyn, David E.

Neves, Luis F. F.

Tan, Yongqiang

Resasco, Daniel E.

Harrison, Roger G.

机构：

[1] Univ Oklahoma, Sch Chem Biol & Mat Engn, Norman, OK 73019 USA

[2] Univ Oklahoma, Carbon Nanotube Technol Ctr, Norman, OK 73019 USA

来源：

NANOTECHNOLOGY | 2007年 / 18卷 / 23期

关键词：

D O I：

10.1088/0957-4484/18/23/235601

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

The objective of this study is to demonstrate the adsorption of horseradish peroxidase ( HRP) on single-walled carbon nanotubes ( SWNTs) using the sodium cholate suspension-dialysis method and to determine the effect of HRP adsorption on the biological activity of HRP and the UV-vis-NIR spectra of the SNWTs. The results indicate that this method results in a stable SWNT-protein suspension with complete retention of enzymatic activity of adsorbed HRP and also retention of a substantial fraction of the NIR absorption at 980 nm. The loading of protein on the SWNTs is high, and the overall yield of preparing the SWNT-protein suspension is also high. This process is promising for preparing SWNT-protein suspensions for biological applications where maintaining protein activity and SWNT absorption are important.

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页数：5

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