Bioluminescence-based assays for detection and characterization of bacteria and chemicals in clinical laboratories

被引:67
作者
Billard, P [1 ]
DuBow, MS [1 ]
机构
[1] Univ Metz, Ctr Environm Sci, F-57040 Metz, France
关键词
bioluminescence; luciferase; biosensor; reporter; phages; bacterial pathogens; chemicals; detection;
D O I
10.1016/S0009-9120(97)00136-7
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Objectives: To survey recent advances in the application of bioluminescence to public health problems. The usefulness of bacterial (lux) and eucaryotic (luc) luciferase genes is presented, along with several examples that demonstrate their value as "reporters" of many endpoints of clinical concern. Conclusions: The development of new technologies for monitoring biological and chemical contaminants is in continuous progress. Recent excitment in this area has come from the use of genes encoding enzymes for bioluminescence as reporter systems. Applications of the recombinant luciferase reporter phage concept now provide a sensitive approach for bacterial detection, their viability, and sensitivity to antimicrobial agents. Moreover, a number of fusions of the lux and luc genes to stress inducible genes in different bacteria can allow a real-time measurement of gene expression and determination of cellular viability, and also constitute a new tool to detect toxic chemicals and their bioavailibility.
引用
收藏
页码:1 / 14
页数:14
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