Synthesis of Lariat-DNA via the chemical ligation of a dumbbell complex

被引：22

作者：

Carriero, S ^{[1
]}

Damha, MJ ^{[1
]}

机构：

[1] McGill Univ, Dept Chem, Montreal, PQ H3A 2K6, Canada

来源：

ORGANIC LETTERS | 2003年 / 5卷 / 03期

关键词：

D O I：

10.1021/ol027227e

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] An efficient synthesis of a medium-sized DNA lariat through the chemical ligation of a Y-shaped dumbbell precursor is described. The methodology requires only commercially available phosphoramidites and reagents and affords regioisomerically pure lariat molecules. Characterization of the lariat by T-m analysis reveals that the molecule displays markedly enhanced thermal stability and unimolecular association-dissociation kinetics, consistent with DNA dumbbell behavior.

引用

收藏

页码：273 / 276

页数：4

相关论文

共 38 条

[1] CHEMICAL SYNTHESIS OF OLIGODEOXYNUCLEOTIDE DUMBBELLS [J].

ASHLEY, GW ;

KUSHLAN, DM .

BIOCHEMISTRY, 1991, 30 (11) :2927-2933

[2]

BLOOMERS MJJ, 1989, BIOCHEMISTRY-US, V28, P7491

[3] Regiospecific solid-phase synthesis of branched oligonucleotides. Effect of vicinal 2',5'- (or 2',3'-) and 3',5'-phosphodiester linkages on the formation of hairpin DNA [J].

Braich, RS ;

Damha, MJ .

BIOCONJUGATE CHEMISTRY, 1997, 8 (03) :370-377

[4] Inhibition of in vitro pre-mRNA splicing in S. cerevisiae by branched oligonucleotides [J].

Carriero, S ;

Braich, RS ;

Hudson, RHE ;

Anglin, D ;

Friesen, JD ;

Damha, MJ .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2001, 20 (4-7) :873-877

[5]

CARRIERO S, 2002, SOLID PHASE SYNTHESI, P1

[6] CONFORMATIONAL PROPERTIES OF BRANCHED RNA FRAGMENTS IN AQUEOUS-SOLUTION [J].

DAMHA, MJ ;

OGILVIE, KK .

BIOCHEMISTRY, 1988, 27 (17) :6403-6416

[7] CHEMICAL SYNTHESIS OF BRANCHED RNA - NOVEL TRINUCLEOSIDE DIPHOSPHATES CONTAINING VICINAL 2'-5' AND 3'-5' PHOSPHODIESTER LINKAGES [J].

DAMHA, MJ ;

PON, RT ;

OGILVIE, KK .

TETRAHEDRON LETTERS, 1985, 26 (40) :4839-4842

[8] AUTOMATED SOLID-PHASE SYNTHESIS OF BRANCHED OLIGONUCLEOTIDES [J].

DAMHA, MJ ;

ZABARYLO, S .

TETRAHEDRON LETTERS, 1989, 30 (46) :6295-6298

[9] Improved MALDI-MS analysis of oligonucleotides through the use of fucose as a matrix additive [J].

Distler, AM ;

Allison, J .

ANALYTICAL CHEMISTRY, 2001, 73 (20) :5000-5003

[10] LARIAT STRUCTURES ARE INVIVO INTERMEDIATES IN YEAST PRE-MESSENGER RNA SPLICING [J].

DOMDEY, H ;

APOSTOL, B ;

LIN, RJ ;

NEWMAN, A ;

BRODY, E ;

ABELSON, J .

CELL, 1984, 39 (03) :611-621

← 1 2 3 4 →