Label-free quantitative cell division monitoring of endothelial cells by digital holographic microscopy

被引：157

作者：

Kemper, Bjoern ^{[1
]}

Bauwens, Andreas ^{[2
]}

Vollmer, Angelika ^{[1
]}

Ketelhut, Steffi ^{[1
]}

Langehanenberg, Patrik ^{[1
]}

Muething, Johannes ^{[2
]}

Karch, Helge ^{[2
]}

von Bally, Gert ^{[1
]}

机构：

[1] Univ Munster, Ctr Biomed Opt & Photon, D-48149 Munster, Germany

[2] Univ Munster, Inst Hyg, D-48149 Munster, Germany

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2010年 / 15卷 / 03期

关键词：

quantitative phase contrast imaging; digital holographic microscopy; live cell imaging; mitosis; cell cycle; cell tracking; PHASE-CONTRAST MICROSCOPY; REFRACTIVE-INDEX; LIVING CELLS; MORPHOMETRY; DYNAMICS;

D O I：

10.1117/1.3431712

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Digital holographic microscopy (DHM) enables quantitative multifocus phase contrast imaging for nondestructive technical inspection and live cell analysis. Time-lapse investigations on human brain microvascular endothelial cells demonstrate the use of DHM for label-free dynamic quantitative monitoring of cell division of mother cells into daughter cells. Cytokinetic DHM analysis provides future applications in toxicology and cancer research. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3431712]

引用

页数：6

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