Fabrication of protein/silica core-shell nanoparticles by microemulsion-based molecular wrapping

被引：64

作者：

Ma, D ^{[1
]}

Li, M ^{[1
]}

Patil, AJ ^{[1
]}

Mann, S ^{[1
]}

机构：

[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England

来源：

ADVANCED MATERIALS | 2004年 / 16卷 / 20期

关键词：

D O I：

10.1002/adma.200400351

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A novel process for wrapping individual myoglobin molecules with an ultrathin shell of silica is reported. Confinement of single protein molecules in microemulsion water droplets followed by the controlled hydrolysis and condensation of tetramethoxysilane at the oil/water interface produces discrete protein/ silica nanoparticles (see Figure) without loss of myoglobin structure and function.

引用

收藏

页码：1838 / +

页数：5

相关论文

共 20 条

[1] REVERSIBLE DENATURATION OF SPERM WHALE MYOGLOBIN .I. DEPENDENCE ON TEMPERATURE PH AND COMPOSITION [J].

ACAMPORA, G ;

HERMANS, J .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1967, 89 (07) :1543-&

[2] SYNTHESIS OF NANOSIZE SILICA IN AEROSOL OT REVERSE MICROEMULSIONS [J].

ARRIAGADA, FJ ;

OSSEOASARE, K .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1995, 170 (01) :8-17

[3] Synthesis of nanosize silica in a nonionic water-in-oil microemulsion: Effects of the water/surfactant molar ratio and ammonia concentration [J].

Arriagada, FJ ;

Osseo-Asare, K .

JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1999, 211 (02) :210-220

[4] Synthesis and microstructure of Pd/SiO2 nanosized particles by reverse micelle and sol-gel processing [J].

Bae, DS ;

Han, KS ;

Adair, JH .

JOURNAL OF MATERIALS CHEMISTRY, 2002, 12 (10) :3117-3120

[5] ENCAPSULATION OF PROTEINS IN TRANSPARENT POROUS SILICATE-GLASSES PREPARED BY THE SOL-GEL METHOD [J].

ELLERBY, LM ;

NISHIDA, CR ;

NISHIDA, F ;

YAMANAKA, SA ;

DUNN, B ;

VALENTINE, JS ;

ZINK, JI .

SCIENCE, 1992, 255 (5048) :1113-1115

[6] A novel and simple alcohol-free sol-gel route for encapsulation of labile proteins [J].

Ferrer, ML ;

del Monte, F ;

Levy, D .

CHEMISTRY OF MATERIALS, 2002, 14 (09) :3619-+

[7]

HAGEN AJ, 1990, BIOTECHNOL BIOENG, V35, P955, DOI 10.1002/bit.260351002

[8] PROTEIN REFOLDING IN REVERSED MICELLES - INTERACTIONS OF THE PROTEIN WITH MICELLE COMPONENTS [J].

HAGEN, AJ ;

HATTON, TA ;

WANG, DIC .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (10) :966-975

[9] IMMOBILIZATION OF MYOGLOBIN IN A MULTILAYER FILM OF 2-DIMENSIONAL POLYMER NETWORK [J].

HAMACHI, I ;

FUJIMURA, H ;

KUNITAKE, T .

CHEMISTRY LETTERS, 1991, (12) :2227-2230

[10] Facile fabrication of polypyrrole nanotubes using reverse microemulsion polymerization [J].

Jang, J ;

Yoon, H .

CHEMICAL COMMUNICATIONS, 2003, (06) :720-721