Degradation of 13C-U-glucose in Sphagnum majus litter:: Responses to redox, pH, and temperature

被引:17
作者
Bergman, I [1 ]
Lundberg, P
Preston, CM
Nilsson, M
机构
[1] Swedish Univ Agr Sci, Dept Forest Ecol, S-90183 Umea, Sweden
[2] Umea Univ, Dept Phys Chem, S-90187 Umea, Sweden
[3] Nat Resources Canada, Pacific Forestry Ctr, Victoria, BC V8Z 1M5, Canada
关键词
D O I
10.2136/sssaj2000.6441368x
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
We studied the utilization of C-13-U-glucose by the microbial community in shallow Sphagnum majus (Russ.) C. Jens. ssp. norvegicum Flatb. litter and its regulation by pH, temperature, and redox conditions. The transformation of C-13-glucose was monitored by solution- and solid-state C-13-nuclear magnetic resonance (MMR) spectroscopy. The aerobic microbial community used the glucose C for respiration and, to a lesser degree, for storage as mannitol, triglycerides, and polysaccharides. Under both aerobic and anaerobic conditions, the allocation of glucose C for storage was greater at pH 6.8 than at 4.3; however, the amount of C used for building new biomass was the same at both pH settings. At 15 degrees C, 15 to 18% of the utilized C under aerobic conditions was found in new microbial biomass: less than the previously reported values of 40 to 72%. This indicates that peat soils may promote significantly different microbial growth patterns from other minerogenic and moor humus soils. The production of mannitol and triglycerides suggests that fungi dominated the microbial community and utilized the glucose under aerobic conditions. Using a combination of solid and liquid NMR techniques we were able, for the first time, to follow the anaerobic pathways of glucose degradation in a natural soil sample. The anaerobic microbial community produced mainly volatile fatty acids (VFA), ethanol, and CO2 from the added glucose, and only minor amounts were converted to methane, storage C, and new microbial biomass. Nuclear magnetic resonance spectroscopy allows nondestructive assays of metabolic events and, therefore, was shown to be an excellent tool for studying the microbial utilization of C-13-glucose in peat.
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页码:1368 / 1381
页数:14
相关论文
共 48 条
[1]  
[Anonymous], EUROPEAN MIRES
[2]   SIGNIFICANCE OF MICROBIAL ACTIVITY IN SOILS AS DEMONSTRATED BY SOLID-STATE C-13 NMR [J].
BALDOCK, JA ;
OADES, JM ;
VASSALLO, AM ;
WILSON, MA .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1990, 24 (04) :527-530
[3]   INCORPORATION OF UNIFORMLY LABELED C-13-GLUCOSE CARBON INTO THE ORGANIC FRACTION OF A SOIL - CARBON BALANCE AND CP MAS C-13 NMR MEASUREMENTS [J].
BALDOCK, JA ;
OADES, JM ;
VASSALLO, AM ;
WILSON, MA .
AUSTRALIAN JOURNAL OF SOIL RESEARCH, 1989, 27 (04) :725-746
[4]   SOLID-STATE CP/MAS C-13 NMR ANALYSIS OF BACTERIAL AND FUNGAL CULTURES ISOLATED FROM A SOIL INCUBATED WITH GLUCOSE [J].
BALDOCK, JA ;
OADES, JM ;
VASSALLO, AM ;
WILSON, MA .
AUSTRALIAN JOURNAL OF SOIL RESEARCH, 1990, 28 (02) :213-225
[5]  
BALDOCK JA, 1992, BIOGEOCHEMISTRY, V16, P1, DOI 10.1007/BF02402261
[6]  
BALDOCK JA, 1995, CARBON FORMS FUNCTIO, P90
[7]   A GENERAL RULE FOR THE ASSIGNMENT OF THE C-13 NMR PEAKS IN FATTY-ACID CHAINS [J].
BENGSCH, E ;
PERLY, B ;
DELEUZE, C ;
VALERO, A .
JOURNAL OF MAGNETIC RESONANCE, 1986, 68 (01) :1-13
[8]  
Bergeson LL, 1998, POLLUT ENG, V30, P29
[9]   Microbial carbon mineralisation in an acid surface peat: effects of environmental factors in laboratory incubations [J].
Bergman, I ;
Lundberg, P ;
Nilsson, M .
SOIL BIOLOGY & BIOCHEMISTRY, 1999, 31 (13) :1867-1877
[10]   MICROBIAL UTILIZATION OF C-14[U]GLUCOSE IN SOIL IS AFFECTED BY THE AMOUNT AND TIMING OF GLUCOSE ADDITIONS [J].
BREMER, E ;
KUIKMAN, P .
SOIL BIOLOGY & BIOCHEMISTRY, 1994, 26 (04) :511-517