Assay of L-ascorbic 2-monophosphate in aquaculture feeds by high-performance anion-exchange chromatography with conductivity detection

L-Ascorbic acid 2-monophosphate (AsMP) was extracted from aquaculture feeds using deaerated, cold water, and the cold extract was subjected immediately to high-performance anion-exchange chromatography with conductivity detection (HPAEC-CD) using gradient elution. The chromatograms of the extracts from three aquaculture feeds showed baseline resolutions of AsMP and recoveries of added AsMP of more than 95%. The detection limit was about 2 ng AsMP (free acid form), which was equivalent to a level of AsMP in feed of 5 mg/kg. The assay for AsMP was direct, rapid, simple, and accurate. (C) 1998 Elsevier Science B.V.