Effect of particulation on the immunogenic and protective properties of the recombinant Bm86 antigen expressed in Pichia pastoris

被引：25

作者：

García-García, JC

Montero, C

Rodríguez, M

Soto, A

Redondo, M

Valdés, M

Méndez, L

de la Fuente, J

机构：

[1] Ctr Genet Engn & Biotechnol, Mammalian Cell Genet Div, Havana, Cuba

[2] Ctr Genet Engn & Biotechnol, Filling & Packaging Div, Havana, Cuba

[3] Natl Parasitol Ctr, Havana, Cuba

来源：

VACCINE | 1998年 / 16卷 / 04期

关键词：

immunogenicity; particulated antigen; Bm86; cattle tick; vaccine;

D O I：

10.1016/S0264-410X(97)80915-2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The recombinant Bm86 tick antigen expressed in Pichia pastoris is obtained in a highly particulated form, as a distinguished feature of this expression system. This particulated protein, the active principle of the recombinant vaccine Gavac(TM) against the cattle tick, have shown high immunogenic and protective properties, probably associated with its own characteristics. To evaluate the effects of particulation on the properties of Bm86, three groups of calves were immunized with particulated or non-particulated recombinant Bm86 and the anti-Bm86 antibody response determined. Animals were challenged with a controlled tick infestation and the protective capacities of both proteins assessed. Humoral immune response and protection in cattle vaccinated with the particulated antigen were higher. These experiments suggested that particulation of the Bm86 expressed in P. pastoris is an important feature for the protective properties of the antigen in vaccine preparations. (C) 1998 Published by Elsevier Science Ltd. All rights reserved.

引用

页码：374 / 380

页数：7

共 18 条

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