Humoral immune response of BALB/c mice to a vaccinia virus recombinant expressing Brucella abortus GroEL does not correlate with protection against a B-abortus challenge

被引：18

作者：

Baloglu, S ^{[1
]}

Toth, TE ^{[1
]}

Schurig, GG ^{[1
]}

Sriranganathan, N ^{[1
]}

Boyle, SM ^{[1
]}

机构：

[1] Virginia Polytech Inst & State Univ, Virginia Maryland Reg Coll Vet Med, Ctr Mol Med & Infect Dis, Blacksburg, VA 24061 USA

来源：

VETERINARY MICROBIOLOGY | 2000年 / 76卷 / 02期

关键词：

Brucella abortus; vaccinia virus; vaccine; immune response; GroEL;

D O I：

10.1016/S0378-1135(00)00231-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This work is a part of an ongoing effort to develop vaccinia virus recombinants expressing various Brucella abortus proteins. The B. abortus groEL gene encoding the antigenic heat shock protein GroEL was subcloned into vaccinia virus via homologous recombination and expression confirmed by Western blotting. Female BALB/c mice inoculated with recombinant vaccinia virus/GroEL produced GroEL and vaccinia virus specific antibodies. Mice were challenged 8 weeks postinoculation with virulent B. abortus strain 2308 and protection measured by the rate of clearance of live Brucella from spleens. Although induction of specific immune response to GroEL and vaccinia virus was demonstrated by the appearance of antibodies in mice, no significant level of protection was demonstrable. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：193 / 199

页数：7

共 24 条

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