A simple and rapid test for differentiation of aerobic from anaerobic bacteria

被引：46

作者：

Karakashev, D ^{[1
]}

Galabova, D ^{[1
]}

Simeonov, I ^{[1
]}

机构：

[1] Bulgarian Acad Sci, Inst Microbiol, Dept Microbial Biochem, BU-1113 Sofia, Bulgaria

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2003年 / 19卷 / 03期

关键词：

aerobes; anaerobes; bacterial respiration; redox indicators;

D O I：

10.1023/A:1023674315047

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A rapid qualitative test is proposed for bacterial respiratory type based on 24 h culturing of bacteria in liquid medium supplemented with a redox indicator: methylene blue or resazurin. Five reference bacterial strains with definite respiratory type as well as nine bacterial isolates from a laboratory digester for methane fermentation were used. Results obtained showed that both indicators can be used for distinction of strict aerobes from other bacterial representatives with definite respiration. In addition, the resazurin is able to differentiate strict anaerobes from microaerophiles and other anaerobes. The main advantages of the methylene blue is that it is a cheap, easily accessible dye, wide-used in microbiological practice and the results obtained with it are more stable over time. It was also noticed that the test with both indicators gave reliable results for the bacterial respiration only when an inoculum up to 48 h old was used.

引用

页码：233 / 238

页数：6

共 15 条

[1]

Angelova M, 1995, OXID COMMUN, V18, P420

[2] IMPROVED IDENTIFICATION OF METHANOGENIC BACTERIA BY FLUORESCENCE MICROSCOPY [J].

DODDEMA, HJ ;

VOGELS, GD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1978, 36 (05) :752-754

[3]

Guerin TF, 2001, LETT APPL MICROBIOL, V32, P340, DOI 10.1046/j.1472-765X.2001.00916.x

[4] The anaerobic degradation of endosulfan by indigenous microorganisms from low-oxygen soils and sediments [J].

Guerin, TF .

ENVIRONMENTAL POLLUTION, 1999, 106 (01) :13-21

[5] Enumeration of acetogens by a colorimetric most-probable-number assay [J].

Harriott, OT ;

Frazer, AC .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) :296-300

[6] STUDIES OF THE MICROAEROPHILIC NATURE OF CAMPYLOBACTER-FETUS SUBSP JEJUNI .1. PHYSIOLOGICAL ASPECTS OF ENHANCED AEROTOLERANCE [J].

HOFFMAN, PS ;

KRIEG, NR ;

SMIBERT, RM .

CANADIAN JOURNAL OF MICROBIOLOGY, 1979, 25 (01) :1-7

[7] Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity [J].

Hugenholtz, P ;

Goebel, BM ;

Pace, NR .

JOURNAL OF BACTERIOLOGY, 1998, 180 (18) :4765-4774

[8] Fate and transformation of thiocyanate and cyanate under methanogenic conditions [J].

Hung, CH ;

Pavlostathis, SG .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 49 (01) :112-116

[9] IMPROVED TECHNIQUE FOR IDENTIFICATION AND ENUMERATION OF METHANOGENIC BACTERIAL COLONIES ON ROLL TUBES BY EPIFLUORESCENCE MICROSCOPY [J].

KATAOKA, N ;

TOKIWA, Y ;

TAKEDA, K .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (12) :3671-3673

[10] Isolation and characterization of refuse methanogens [J].

Ladapo, JA ;

Barlaz, MA .

JOURNAL OF APPLIED MICROBIOLOGY, 1997, 82 (06) :751-758

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